Biotinylated dextran amine tracing of nerve tracts determines regeneration of corticospinal tracts after neural stem cell transplantation  

作　　者：Jun Wu Fenghua Chen Mingyu Zhang Tao Song Jinfang Liu Jiasheng Fang 

机构地区：[1]Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China

出　　处：《Neural Regeneration Research》2011年第28期2183-2187,共5页中国神经再生研究（英文版）

基　　金：Hunan Province Science and Technology Department Plan,No.2009JT3051

摘　　要：We investigated regeneration of the corticospinal tract and rearrangement of corticospinal nerves after spinal cord injury by biotinylated dextran amine （BDA） nerve tract tracing after neural stem cell transplantation. Neural stem cell transplantation increased motor function scores of rats at 3 weeks after spinal cord transection injury at the thoracic 10 segment. A proportion of BDA-labeled corticospinal tract regenerated through the spinal cord injury site at 12 weeks after transplantation. Electron microscopy revealed that the regenerated BDA-labeled nerve terminals formed new synaptic connections with neurons at the distal end of the injured site. These findings indicate that BDA nerve tract tracing effectively provides anatomic and morphological evidence of recovery after spinal cord injury.We investigated regeneration of the corticospinal tract and rearrangement of corticospinal nerves after spinal cord injury by biotinylated dextran amine （BDA） nerve tract tracing after neural stem cell transplantation. Neural stem cell transplantation increased motor function scores of rats at 3 weeks after spinal cord transection injury at the thoracic 10 segment. A proportion of BDA-labeled corticospinal tract regenerated through the spinal cord injury site at 12 weeks after transplantation. Electron microscopy revealed that the regenerated BDA-labeled nerve terminals formed new synaptic connections with neurons at the distal end of the injured site. These findings indicate that BDA nerve tract tracing effectively provides anatomic and morphological evidence of recovery after spinal cord injury.

关 键 词：nerve tract tracing neural stem cells TRANSPLANTATION spinal cord injury corticospinal tract biotinylated dextran amine neural regeneration 

分 类 号：Q813[生物学—生物工程] Q959.804