Establishment of an animal model of extra-vertebral foramen cervical nerve entrapment by local electrical stimulation and selection of suitable stimulation parameters  

作　　者：Jinwu Wang Juliet Hou Qi Li Jian Tang Biyu Rui Shangchun Guo Haitao Jiang Cunyi Fan You Wang Zhenan Zhu Kerong Dai Guang H Yue 

机构地区：[1]Department of Orthopedic Surgery, the Ninth People＇s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China [2]Department of Physical Medicine ＆ Rehabilitation, the Cleveland Clinic, Cleveland, Ohio 44195, USA [3]Department of Orthopaedic Surgery, the Sixth Municipal People＇s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China [4]Biomedical Engineering, the Cleveland Clinic, Cleveland, Ohio 44195, USA

出　　处：《Neural Regeneration Research》2011年第29期2284-2289,共6页中国神经再生研究（英文版）

基　　金：the National Natural Science Foundation of China,No. 81171707;the Major State Basic Research Program of China,No.2012CB933600;Shanghai Pujiang Program,No.11PJD016;China Postdoctoral Science Foundation,No. 20090460629;Fund for Key Disciplines of Shanghai Municipal Education Commission,No.J50206

摘　　要：A rat model of extra-vertebral foramen cervical nerve entrapment was established according to the following parameters： stimulation intensity 20 V; frequency 50 Hz; pulse width 200 μs; duration 333 ms/s for a total of 8 hours. After the electrical stimulation, rats exhibited mild muscle fiber atrophy, mild inflammatory exudates, connective tissue local fibrosis and chondrocyte metaplasia. Mean muscle fiber cross-sectional area was reduced. The nerve myelin sheath continuity was partially demyelinated. The microstructure of nerve cells was disrupted and these symptoms worsened with prolongation of the stimulation. The shoulder, neck and upper extremity muscles on the tested side demonstrated positive sharp waves and fibrillations. The severity increased with continuation of the stimulation. High amplitude and polyphasic motor unit potentials gradually appeared. Similar findings were seen in the contralateral side, but at a less severe level.A rat model of extra-vertebral foramen cervical nerve entrapment was established according to the following parameters： stimulation intensity 20 V; frequency 50 Hz; pulse width 200 μs; duration 333 ms/s for a total of 8 hours. After the electrical stimulation, rats exhibited mild muscle fiber atrophy, mild inflammatory exudates, connective tissue local fibrosis and chondrocyte metaplasia. Mean muscle fiber cross-sectional area was reduced. The nerve myelin sheath continuity was partially demyelinated. The microstructure of nerve cells was disrupted and these symptoms worsened with prolongation of the stimulation. The shoulder, neck and upper extremity muscles on the tested side demonstrated positive sharp waves and fibrillations. The severity increased with continuation of the stimulation. High amplitude and polyphasic motor unit potentials gradually appeared. Similar findings were seen in the contralateral side, but at a less severe level.

关 键 词：chronic neck-shoulder pain electrical stimulation extra-vertebral foramen cervical nerve entrapment syndrome animal model 

分 类 号：S823.2[农业科学—畜牧学] Q421[农业科学—畜牧兽医]