兔膝骨关节炎合并骨质疏松症动物模型的建立  

Establishment of a rabbit model of knee joint osteoarthritis combined with osteoporosis

作　　者：周君[1,2] 何成奇[1,2] 郭华[1,2] 夏璐[1,2] 覃渝茜[1,2] 刘慧芳[1,2] 

机构地区：[1]康复医学四川省重点实验室,四川省成都市610041 [2]四川大学华西医院康复科,四川省成都市610041

出　　处：《中国组织工程研究与临床康复》2011年第41期7639-7642,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:临床发现同时患有骨质疏松症和骨关节炎患者占相当大的比例,而且目前对骨质疏松与骨关节炎相互关系的认识不一致。目的:建立骨关节炎合并骨质疏松症的动物模型。方法:将14只新西兰大白兔随机等分为模型组和正常组。模型组新西兰大白兔行双侧卵巢切除,正常组新西兰大白兔不作任何处理。结果与结论:去除卵巢10周后,模型组大白兔关节软骨出现明显的退变,血清雌二醇、股骨骨密度水平较正常组显著下降(P<0.01),而关节软骨Mankin评分比正常组显著增高(P<0.01);且软骨Mankin评分与骨密度和血清雌二醇呈负相关,而骨密度与血清雌二醇水平呈正相关,表明实验成功建立了兔膝骨关节炎合并骨质疏松症动物模型。BACKGROUND：Clinical studies have shown that there are a large proportion of patients suffering from osteoarthritis（OA） combined with osteoporosis（OP）.Now the relationship between OA and OP is disputed.So an animal model of OA combined with OP is beneficial to prevention and treatment of OA combined with OP and also facilitates to understand the relationship between OA and OP.OBJECTIVE：To establish an animal model of OA combined with OP.METHODS：Fourteen white New Zealand rabbits were randomly divided into a normal control group（NC group） and an ovariectomy group（OVX group）.The OVX group rabbits underwent bilateral ovariectomy.The NC group rabbits received no treatments.RESULTS AND CONCLUSION：At 10 weeks after OVX,the OVX group rabbits showed obvious articular cartilage degeneration and serum estrogen and femoral bone mineral density were significantly decreased,while articular cartilage Mankin scores were significantly increased compared with the NC group（P 0.01）.Articular cartilage Mankin scores were negatively correlated with serum estrogen,while bone mineral density was positively correlated with serum estrogen.These results showed that a rabbit model of OA combined with OP of the knee joint can be successfully established by OVX.

关 键 词：骨质疏松 骨关节炎 动物模型 组织构建 兔组织工程 

分 类 号：R318[医药卫生—生物医学工程]