人脐血间充质干细胞对T淋巴细胞旁分泌的免疫调节作用  

Immunoregulatory effect of human umbilical cord blood mesenchymal stem cells on proliferation of T lymphocytes through paracrine mechanism

作　　者：徐丽南[1] 姚志成[2] 林楠[2] 胡昆鹏[2] 钟跃思[2] 

机构地区：[1]中山大学附属第一医院妇产科,广东省广州市510080 [2]中山大学附属第三医院肝胆外科,广东省广州市510630

出　　处：《中国组织工程研究与临床康复》2011年第40期7463-7466,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学青年基金(81000674);广东省科技计划项目(2010B031600313)~~

摘　　要：背景:相关研究表明脐血间充质干细胞具有一定的免疫调节作用,但具体机制不详。目的:观察脐血源间充质干细胞通过旁分泌机制对T淋巴细胞增殖的影响。方法:分离正常分娩产妇脐血间充质干细胞和健康志愿者外周血T淋巴细胞,按不同比例建立脐血间充质干细胞与T淋巴细胞非接触共培养体系,以单独培养T淋巴细胞作为对照组。结果与结论:脐血间充质干细胞形态学呈现成纤维梭型细胞样,呈旋涡状团集生长。流式细胞仪检测脐血间充质干细胞表面标记物:CD29(+),CD44(+),CD34(-),CD45(-),HLA-DR(-);与对照组相比,共培养组可明显抑制植物血凝素刺激T淋巴细胞的增殖作用(P<0.05),且呈剂量依赖性;ElISA法检测共培养组分泌的白细胞介素10水平较对照组明显升高(P<0.05);中和试验后脐血间充质干细胞对T淋巴细胞增殖的抑制作用明显减弱。结果说明脐血间充质干细胞可明显抑制异体外周血T淋巴细胞的增殖,可能是通过旁分泌白细胞介素10达到负向免疫调节作用。BACKGROUND： Studies have shown that umbilical cord blood mesenchymal stem cells （UCB-MSCs） have a certain role in immune regulation, but the exact mechanism is unknown. OBJECTIVE： To study the effect of UCB-MSCs on T lymphocytes by paracrine mechanism. METHODS： We isolated the UCB-MSCs and T lymphocytes from the umbilical cord blood and peripheral blood. Indirect coculture system of UCB-MSCs and T lymphocytes with different proportion was constructed. The group of coculture acted as experiment group, the group of monoculture T lymphocytes as control group. RESULTS AND CONCLUSION： UBC-MSCs presented a shuffle-type growth which liked the fibroblast in morphology; the flow cytometry showed the results of cellular surface marks： CD29（＋）, CD44（＋）, CD34（-）, CD45（-）, HLA-DR（-）. Compared with the control group, the experiment group could dramatically inhibit the proliferation of peripheral blood T lymphocytes stimulated by phytohemagglutinin in a dose-dependent manner （P 0.05）; the concentration of interleukin-10 secreted by UCB-MSCs was significantly higher in the experiment group than the control group （P 0.05）. The ability of UBC-MSCs to inhibit the proliferation of T lymphocytes was sharply impaired by neutralization experiments. UCB-MSCs can dramatically inhibit the proliferation of peripheral blood T lymphocytes, which may depend on paracrine interleukin-10 to play an immunoregulatory role.

关 键 词：免疫调节 脐血间充质干细胞 T淋巴细胞 旁分泌 白细胞介素10 

分 类 号：R394.2[医药卫生—医学遗传学]