肾移植患者外周血中的CD4~＋ CD25~＋ CD127^(low/-)调节性T细胞  

CD4^+ CD25^+ CD127^(low/-) regulatory T cells in the peripheral blood of kidney transplantation patients

作　　者：陈远林[1] 曾钰钦[2] 

机构地区：[1]中南大学湘雅二医院检验科,湖南省长沙市410011 [2]中南大学湘雅医学院检验系2005级,湖南省长沙市410010

出　　处：《中国组织工程研究与临床康复》2011年第44期8209-8213,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:越来越多的实验在分析免疫耐受标志,以期能够更好地辅助患者进行移植后免疫抑制治疗。目的:分析肾移植后患者外周血中CD4+CD25+CD127low/-调节性T细胞在肾移植免疫耐受中的作用。方法:采集62例肾移植后患者(急性排斥反应组22例,移植稳定组40例)及20例健康对照者的外周抗凝血,经免疫染色,应用流式细胞仪分析CD4+CD25+CD127low/-调节性T细胞所占CD4+T细胞百分含量,同时采用ELISA方法检测患者血清中白细胞介素2和白细胞介素10的质量浓度。结果与结论:移植稳定组中CD4+CD25+CD127low/-调节性T细胞所占CD4+T细胞百分含量显著高于健康对照组和急性反应排斥组(P<0.01);CD4+CD25+CD127low/-调节性T细胞百分含量与白细胞介素2呈显著负相关(P<0.05),与白细胞介素10呈显著正相关(P<0.01)。提示CD4+CD25+CD127low/-调节性T细胞在肾移植后免疫耐受的机制中发挥了一定作用。BACKGROUND： More and more studies have been performed to analyze immune tolerance signs to better assist patients to undergo immunosuppressive therapy after transplantation. OBJECTIVE： To investigate the role of CD4＋ CD25＋ CD127low/- regulatory T cells in the peripheral blood of renal transplant patients in the immune tolerance following renal transplantation. METHODS： Peripheral blood from 62 patients with primary glomerulonephritis who received renal transplantation （22 included in the acute rejection group and 40 in the transplantation stability group） and 20 healthy controls was harvested, anticoagulated and stained. The proportion of CD4＋ CD25＋ CD127low/- regulatory T cells in the CD4＋ T cells was analyzed through the use of flow cytometer. Serum concentration of interleukin 2 and 10 was detected using enzyme-linked immunosorbent assay. RESULTS AND CONCLUSION： The proportion of CD4＋ CD25＋ CD127low/- regulatory T cells in the CD4＋ T cells was significantly higher in the transplantation stability group than that in the healthy control group and acute rejection group （P 0.01）. The proportion of CD4＋ CD25＋ CD127low/- regulatory T cells in the CD4＋ T cells was significantly negatively correlated with interleukin 2 （P 0.05） but it was significantly positively correlated with interleukin 10 （P 0.01）. These findings suggest that CD4＋ CD25＋ CD127low/- regulatory T cells exert some effects in the mechanism underlying immune tolerance after renal transplantation.

关 键 词：肾移植 CD4+CD25+CD127low/-调节性T细胞 白细胞介素2 白细胞介素10 免疫耐受 

分 类 号：R617[医药卫生—外科学]