六神丸逆转K562/DOX细胞阿霉素耐药的研究  

Study of Liushen Wan changeovering drug resistance of K562/DOX to doxorubicine

作　　者：李海燕[1] 

机构地区：[1]广州中医药大学,广州510006

出　　处：《中药药理与临床》2011年第5期3-5,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：广东省中医药管理局中医药强省项目(2008158)

摘　　要：目的:探讨六神丸对阿霉素耐药细胞株K562/DOX的耐药逆转作用。方法:MTT法检测单独应用阿霉素或阿霉素联合六神丸含药血清对K562/DOX细胞的生存率的影响;RT-PCR检测MDR1 mRNA表达;Western blot检测MDR1蛋白的表达。结果:阿霉素与六神丸含药血清联合作用:①细胞抑制率最高,最高值出现在72h(49.4%),且与对照组和单独应用阿霉素组相比有显著性差异;②48h细胞MDR1 mRNA表达明显降低,与单独应用阿霉素组相比有显著性差异;③48h细胞MDR1蛋白表达下降,且与对照组和单独应用阿霉素组相比有显著性差异。结论:六神丸可能通过下调MDR1表达水平进而逆转K562/DOX细胞对阿霉素的耐药性,增加肿瘤耐药细胞对化疗药物的敏感性。Objective：Discuss the effect of Liushen Wan changeover the drug resistance of K562/DOX.Method： Detect survival rate of using doxorubicine or doxorubicine and Liushengwan in MTT;detect expression of MDR1 mRNA in RT-PCR;detect protein expression of MDR1 in Western blot.Result： Compare with control group,the inhibition ratio was not changing only using doxorubicine;but the livability,expression of MDR1 mRNA and detect protein expression of MDR1 were reduced.Conclusion： Liushenwan maybe reduce expression of MDR1 and then changeover drug resistance of K562/DOX to doxorubicine;increase the sensibility of the cell to chemotherapeutics resistance.

关 键 词：六神丸 K562/DOX细胞 MDR1基因 

分 类 号：R285[医药卫生—中药学]