黄花苜蓿愈伤组织诱导及分化培养条件的研究  

Study on Culture Conditions for Callus Induction and Differentiation of Medicago falcata

作　　者：伊风艳[1] 石凤翎[1] 展春芳[1] 姜圆圆[1] 

机构地区：[1]内蒙古农业大学生态环境学院,内蒙古呼和浩特010019

出　　处：《中国草地学报》2011年第6期14-20,共7页Chinese Journal of Grassland

基　　金：国家科技部"863"计划项目(2009AA10Z108);内蒙古社会发展领域科技计划项目(20082002)

摘　　要：以黄花苜蓿子叶和下胚轴为外植体,研究不同激素配比对黄花苜蓿愈伤组织诱导及分化的影响,结果表明:MS+2,4-D 2mg/L+6-BA0.5mg/L+蔗糖3%+琼脂0.7%为适宜的愈伤组织诱导培养基;MS+KT0.5mg/L+NAA0.1mg/L+蔗糖2%+琼脂0.7%为适宜的分化培养基;生根培养基为:1/2MS+NAA 0.1mg/L+蔗糖2%+琼脂0.7%。下胚轴是诱导愈伤及分化的最佳外植体,诱导率可达100%,分化率可达80%。The cotyledon and hypocotyls of Medicago falcata L. were used as explants to study the effect of plant hormone ratio on induction and differentiation of callus of Medicago falcata L.. The results showed that： MS＋2,4-D 2 mg/L ＋6-BA 0.5 rag/L＋ sucrose 3%＋ager 0.7% was suitable for callus induction. MS＋KT 0.5 mg/L ＋NAA 0.1 mg/L ＋ sucrose 2~＋ager 0.7~ was suitable for callus differentiation. 1/2MS＋ NAA 0.1 mg/L ＋ sucrose 2%＋ager 0.7% was suitable for rooting culture. Hypocotyls were the optimal explants of Medicago falcata L. for callus induction and differentiation. Induction rate can amount to 100% and differentiation rate to 80%.

关 键 词：黄花苜蓿 愈伤组织 诱导率 分化率 

分 类 号：Q813.1[生物学—生物工程]