紫外分光光度法测定参七复脉片中总皂苷含量  

Determination of total triterpenoid saponin Shenqifumai tablets by UV spectrophotometry

作　　者：刘凯[1] 陈江[1] 肖引[1] 彭国华[1] 李广琪[1] 陈芳[1] 李勃[1] 

机构地区：[1]武警陕西总队医院药剂科,西安710054

出　　处：《武警医学》2011年第11期930-931,共2页Medical Journal of the Chinese People's Armed Police Force

基　　金：武警部队后勤部课题资助项目(编号wJHQ2008-05)

摘　　要：目的建立紫外分光光度法测定参七复脉片中总皂苷含量的方法。方法以人参皂苷Re为对照品,以5%香草醛-冰醋酸溶液和高氯酸混合溶液为显色剂,测定波长为560 nm。结果人参皂苷Re浓度在3.04～15.2μg范围内,吸收度与浓度呈良好的线性关系(r=0.9993);平均加样同收率为97.64%(RSD为0.65%)。结论所建立之方法准确可靠、灵敏度高、专属性强,可用于参七复脉片的质量控制。Objective To establish an ultraviolet spectrophotometric determination method for total triterpenoid saponin in Shenqifumai tablets. Methods The content was determined with 5% vanillin - glacial acetic acid - sulphuric acid - perchloric acid mixture as the color developing reagent and ginsenoside Re as the standard sample. Results Ginsenoside Re had the linear range of 3.04 - 15.2μg（r = 0. 9993）. The average recovery was 97.64% （ RSD =0.65% ）. Conclusions This method is reliable , accurate and specific. It can be used for quality control of Shenqifumai tablets.

关 键 词：参七复脉片 总皂苷 紫外分光光度法 含量测定 

分 类 号：R284[医药卫生—中药学]