高压氧预处理对高原肺水肿大鼠肺水通道蛋白-5的影响  

Effect of hyperbaric oxygen preconditioning on expression of lung aquaporin - 5 in rat models of high altitude pulmonary edema

作　　者：李茁[1] 赵立明[1] 王丛[1] 刘雪华[1] 吕春凤[2] 高春锦[1] 

机构地区：[1]首都医科大学附属北京朝阳医院高压氧科,100020 [2]北京航天总医院,100076

出　　处：《武警医学》2011年第11期938-942,共5页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的观察高压氧预处理(hyperbaric oxygen preconditioning,HBO-PC)对高原肺水肿(high-altitude pulmonaryedema,HAPE)大鼠肺水通道蛋白-5(AQP5)的影响。方法 38只Wistar大鼠,分对照组、HAPE模型组(HAPE组)及高压氧预处理组(HBO组)。制作HAPE模型后,分别以Western Blot法及Real-Time PCR法观察各组大鼠肺AQP5蛋白、基因表达,同时观察肺水含量及病理变化。结果 HAPE组肺湿/干比明显高于Control组;HBO组肺损伤积分明显低于HAPE组;HAPE组AQP5及AQP5mRNA较Control组显著降低,HBO组AQP5及AQP5mRNA均明显高于HAPE组;肺组织损伤与肺AQP5表达有密切的相关关系,且呈负相关。结论 AQP5表达降低可能参与了HAPE的发病机制;HBO-PC能上调AQP5表达,具有预防和减轻HAPE的作用。Objective To investigate the possibility of applying hyperbaric oxygen preconditioning （ HBO - PC） to reduce or prevent high -altitude pulmonary edema （HAPE） and to find out if aquaporin- 5 （AQP5） is involved in HAPE pathogenesis using a rat model. Methods Rats were divided into three groups ： control, HBO and HAPE. Western blotting and real - time PCR were used to analyze the expression of AQP5 in the lung. The wet - to - dry weight ratio （ W/D weight ratio） and morphology of the lung were also examined. Results An increased lung W/D weight ratio was demonstrated in the HAPE group compared with the control group. Marked interstitial edema in all lung sections in the HAPE group, and small changes in 10 of the 13 sections were present in the HBO group. Lung- injury scores in the HBO group were much lower than those in the HAPE group. AQP5 in the HAPE group decreased significantly at the protein and gene level, compared with those in the control group. Protein and mRNA expression of AQP5 in the HBO group were much higher than those in the HAPE group. Lung injury in the HAPE rats was related positively to the AQP5 expression in the lung. Conclusions HBO - PC can alleviate lung injury in rats caused by high - altitude hypobaric hypoxia and reduce HAPE incidence. HAPE in rats is associated with a downregulation of expression of AQP5 in the lung. This downregulation can be attenuated by HBO - PC. This study is the first report to introduce HBO - PC in the prevention of HAPE and the first investigation of the expression of AQP5 in a rat model of HAPE.

关 键 词：高原肺水肿 高压氧预处理 水通道蛋白-5 肺组织病理 肺湿/干比值 

分 类 号：R563.19[医药卫生—呼吸系统]