乙肝病毒外膜大蛋白检测及其对判定HBV DNA复制的意义  

Detection of serum hepatitis B virus large envelope protein and its relationship with viral replication

作　　者：张红娟[1] 崔辰莹[1] 张洁[1] 王丽伟[1] 张雪丽[1] 

机构地区：[1]秦皇岛市第一医院检验科,066000

出　　处：《武警医学》2011年第11期943-945,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探讨血清乙肝病毒外膜大蛋白(LHBs)检测对于判定HBV DNA复制的临床意义。方法采用酶联免疫方法检测LHBs、Pre-SI和HBV M,采用实时荧光定量PCR法检测HBV DNA。结果 (1)在HBeAg阳性血清中,HBV DNA与LHBs、Pre-S1之间的阳性率差异均无统计学意义(P>0.05);(2)在HBeAg阴性血清中,HBV DNA与LHBs的阳性率差异无统计学意义(P>0.05),HBV DNA与Pre-S1之间的阳性率差异有统计学意义(P<0.05);(3)90份乙肝患者血清中LHBs水平与HBV DNA拷贝数呈良好相关性(r=0.918),Pre-S1水平与HBV DNA拷贝数的相关系数为0.765结论血清LHBs水平能反映HBV DNA复制程度,其与HBV DNA的相关性优于Pre-S1,可作为评判HBV DNA复制新的血清学指标。Objective To study the clinical significance of HBV large envelope protein in diagnosing viral replication in chronic hepatitis B patients. Methods EI,ISA was used to measure the level of serum LHBs and pre - S1. HBV markers were detected by enzyme linked immunosorbent assay. HBV DNA was detected using quantitative fluorescent PCR . Results In terms of the positive rate, there was no difference between HBV DNA and LHBs,but the difference was significant between HBV DNA and pre -S1 as in HBeAg positive samples （P 〉 0. 05 ）. In HBeAg negative samples, both the posivite rate of HBV DNA and that of LHBs were 72. 22%. The difference between the rate of HBV DNA and that of pre - S1 was significant （ P 〈 0. 05 ）. LHBs levels were correlated with the number of HBV DNA copies（ r = 0. 918 ） , and the correlation between HBV DNA and pre - S1 was 0. 765. Conclusions The level of serum LHBs can be used to estimate the state of viral replication and the correlation is superior to that of pre - S1. So it can be used as a new serological marker to detect viral replication.

关 键 词：乙肝病毒 外膜大蛋白 HBV DNA 

分 类 号：R446.11[医药卫生—诊断学]