雷氏珍菊降压片的HPLC指纹图谱研究  

Establishment of the Fingerprint of Leishi Brand Zhenju Jiangya Tablet by HPLC

作　　者：徐晓英[1] 唐宇伟[1] 黄德杰[1] 张国明[1] 

机构地区：[1]上海市中药研究所,上海201401

出　　处：《中国现代中药》2011年第11期39-42,共4页Modern Chinese Medicine

基　　金：上海市技术创新体系建设项目[上海市经委2004(沪创新J-7)]

摘　　要：目的:探索建立雷氏珍菊降压片的指纹图谱质量控制方法。方法:采用RP-HPLC梯度洗脱法测定珍菊降压片的指纹图谱,并运用国家药典委员会发布的《中药色谱指纹图谱相似度评价系统》软件分析比较其相似度。结果:初步建立了含有10个特征峰的雷氏珍菊降压片高效液相指纹图谱,并归属了其中的盐酸可乐定、氢氯噻嗪、芦丁和蒙花苷峰;14批样品相似度系数均大于0.989。结论:RP-HPLC(DAD)法指纹图谱特征性较强、重复性好,可用于珍菊降压片的质量控制。Objective： To establish the fingerprint of Leishi Brand Zhenju Jiangya Tablet. Methods： A fingerprinting of Leishi Brand Zhenju Jiangya Tablet was invested by RP-HPLC with a gradient dution mode. , the simalarity was analysed by Fingerprint Similarity Evaluation Software. Results： The fingerprinting with 10 characteristic peaks,among of which peak 4,5,6 and 10 identified as Clonidine hydrochloride, Hydrochlorothiazide, Rutin and Linaring. The similarity of 14 samples are all higher than 0. 989. Conclusion： The fingerprint toothed established show to be easy, repeatable and sensitive to distinguish the samples tested.

关 键 词：雷氏珍菊降压片 指纹图谱 RP—HPLC 

分 类 号：R944[医药卫生—药剂学]