河北省赵县某村单采血浆还输血细胞献血员慢性HBV和/或HCV感染与IL-10-1082和IL-10-592基因多态性的关系  

Association of IL-10-1082 and IL-10-592 polymorphisms with chronic hepatitis B and/or hepatitis C virus infection among plasma donors in a rural area of Hebei Province,China

作　　者：高秋菊[1] 张世勇[2] 吴丽红[1] 贾敏[3] 米裕[1] 刘殿武[4] 

机构地区：[1]白求恩军医学院预防医学教研室,石家庄050081 [2]石家庄市疾病预防控制中心 [3]河北省人民医院 [4]河北医科大学公共卫生学院

出　　处：《卫生研究》2011年第6期709-713,共5页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No.30972516)

摘　　要：目的研究HBV和/或HCV感染者IL-10-1082和IL-10-592基因多态性(SNP),探讨其免疫遗传机制。方法对河北赵县某村HBV和/或HCV感染者及对照共277人采集空腹静脉血。用ELISA法检测抗-HBV、抗-HCV生物标志物,筛出HBV重叠HCV感染79例、单纯HBV感染69例、HCV感染55例和对照74例。用RT-n PCR检测HCV RNA,Beckman LX-20全自动生化仪检测肝功能丙氨酸氨基转移酶(ALT),SSP-PCR和RFLP-PCR技术检测IL-10-1082和IL-10-592 SNP,分析IL-10-A1082G、IL-10-A592C与HBV和/或HCV感染、HCV病毒复制和ALT异常的关系。结果①HBV和/或HCV感染者IL-10-1082AA基因型频率明显高于对照组,IL-10-1082AG基因型频率明显低于对照组(χ2=13.05,P=0.04),携带IL-10-1082AA基因型者感染HBV、HCV和HBV重叠HCV感染的风险是IL-10-1082AG基因型者的2.29(1.10～4.78)、2.34(1.07～5.10)、2.56倍(1.25～5.21);IL-10-1082等位基因频率与不同感染类型间未见统计学关联(χ2=4.65,P=0.20)。IL-10-592位点SNP与不同感染类型间无明显统计学关联(χ2=2.83,P=0.83;χ2=1.10,P=0.78)。②HCV复制组IL-10-1082AA基因型和IL-10-1082A等位基因频率明显高于无病毒复制组,IL-10-1082AG基因型和IL-10-1082G等位基因频率明显低于无病毒复制组(χ2=12.27,P=0.00;χ2=5.36,P=0.02),OR=3.36(1.67～6.76)、1.67(1.08～2.57)。IL-10-592位点SNP与体内HCV病毒复制无明显统计关联(χ2=2.10,P=0.35;χ2=1.88,P=0.17)。③IL-10-1082位点SNP与ALT异常无统计学关联(χ2=3.25,P=0.20;χ2=1.79,P=0.18)。ALT≥80U/L组IL-10-592AC基因型频率明显高于、IL-10-592AA基因型频率明显低于肝功<80U/L组(χ2=6.32,P=0.04),OR=2.83倍(1.26～6.37),IL-10-592A/C等位基因与肝功异常无明显统计关联(χ2=2.30,P=0.09)。结论 IL-10-1082基因型与HBV和/或HCV感染类型、病毒复制有明显的统计学关联,IL-10-1082AA基因型能增加感染者慢性化风险,IL-10-1082AG基因型则降低其风险。IL-10-592位点多态性与HBV/HCV感染者的肝功损伤有一定联系,IL-10-5Objective To study the relationship between the polymorphisms of interleukin-10 gene at position-1082,-592（IL-10-1082,IL-10-592） and chronic HBV and/or HCV infection.Methods 277 subjects（79 chronic HCV/HBV co-infection,69 chronic HBV infection,55 chronic HCV infection and 74 control subjects） were recruited from plasma donors in a rural area of Hebei Province,China.The single nucleotide polymorphism of IL-10-1082 and IL-10-592 was investigated by sequence specific primer-PCR（SSP-PCR） and restricted fragment long polymorphism-PCR（RFLP-PCR）.Hepatocellular injury was detected by alanine aminotransferase（ALT） with Beckman LX-20 analyzer.The presence of hepatitis C viral particles in serum was determined by RT-nPCR.Results ① Chronic HCV or HBV infection or HCV/HBV co-infection was associated with higher frequency of IL-10-1082 AA genotype [χ2=13.05,P=0.04;OR=2.29（1.10-4.78）,2.34（1.07-5.10）,2.56（1.25-5.21）].There was no significant association of IL-10-1082 allele frequency with HCV or HBV infection or HCV/HBV co-infections（χ2=4.65,P=0.20）.There was no significant association of IL-10-592 genotype and allele frequency with chronic HBV and/or HCV infection（χ2=2.83,P=0.83;χ2=1.10,P=0.78）②There were obvious associations of higher IL-10-1082 AA genotype and A allele frequency with HCV replication [（χ2=12.27,P=0.00;χ2=5.36,P=0.02）,OR=3.36（1.67-6.76） and 1.67（1.08-2.57）].The polymorphism of IL-10-592 was not associated with HCV RNA replication（χ2=2.10,P=0.35;χ2=1.88,P=0.17）.③The polymorphism of IL-10-1082 was not significantly associated with abnormal ALT（χ2=3.25,P=0.20;χ2=1.79,P=0.18）.Abnormal serum ALT level was associated with IL-10-592 AC genotype [（χ2=6.32,P=0.04）,OR=2.83（1.26-6.37））,but the IL-10-592 A/C allele frequency was not associated with abnormal serum ALT level（χ2=2.30,P=0.09）.Conclusion These results suggested that the polymorphism of IL-10-1082 appears to have some influences on the chronic infection of HCV and/or HBV

关 键 词：病毒性肝炎 HBV感染 HCV感染 IL-10 单核苷酸多态性 

分 类 号：R512.6[医药卫生—内科学]