孕哺期铅暴露对仔鼠海马组织中Aβ_(40)表达的影响  

Effects of maternal lead exposure on the expression of Aβ_(40) in the hippocampus of the filial mice

作　　者：李宁[1] 杨国俊[2] 乔明武[1] 郑艳涛[3] 李文杰[3] 

机构地区：[1]河南农业大学食品科学技术学院,郑州450002 [2]河南职工医学院 [3]郑州大学公共卫生学院

出　　处：《卫生研究》2011年第6期779-781,共3页Journal of Hygiene Research

摘　　要：目的探讨母体铅染毒对子一代海马组织中β淀粉样蛋白40(Aβ40)表达的影响。方法雌性小鼠自妊娠第1天开始经饮水染铅(0.3、1.0及3.0g/L,对照组饮蒸馏水)至仔鼠出生后21d断乳为止。随机抽取各组仔鼠,在出生后第7、14、21天分别测其血液和海马组织中铅的含量;在出生后第21天时,采用免疫组化方法测定各组海马组织中Aβ40的表达。结果孕哺期不同剂量铅暴露后,7、14和21天的仔鼠血铅、海马铅水平均明显高于对照组,差异有统计学意义(P<0.05)。免疫组化染色结果显示,Aβ40免疫组化阳性反应主要定位于胞浆和胞膜;各个铅暴露组仔鼠海马的CA1区域Aβ40免疫组化阳性反应物的面密度显著高于对照组,差异均有统计学意义(P<0.05);与对照组相比,各个铅暴露组仔鼠海马的CA1区域Aβ40免疫组化阳性反应物的平均灰度值明显降低,差异有统计学意义(P<0.05)。结论母体铅暴露使铅在仔鼠体内蓄积,增强了仔鼠海马组织中Aβ40的聚集,干扰了正常的海马神经元的功能,进而可能影响其学习记忆功能。Objective To investigate the lead exposure of pregnant and lactating mice on the expression of β-amyloid 40（Aβ40） in the hippocampus of filial mice,in order to reveal the mechanism of neurotoxicity induced by lead.Methods Lead exposure was conducted through freely drinking lead acetate solution with dosages of 0.3g/L,1.0g/L and 3.0g/L respectively.The contents of lead in blood and hippocampus of the filial mice（10 mice in each group） were determined 7,14 and 21 days after their birth.The expression of Aβ40 in hippocampus of all filial mice in various dosage groups was determined on the 21st day after birth by immunohistochemical assay.Results The lead levels in blood and hippocampus of 7,14 and 21 day-old filial mice in lead exposure groups were higher than those in control group（P0.05）.The results of immunohistochemistry showed that Aβ40 was mainly located in the cytoplasm.The area density of positive immune reaction of Aβ40 in CA1 field of hippocampus in three lead exposure groups were higher than that in the control group（P0.05）,but the average gray value of Aβ40 positive immune reaction in CA1 field of hippocampus in lead exposure groups was lower than that in control group（P0.05）.Conclusion Maternal lead exposure may induce the accumulation of lead in filial mice,and enhance the aggregation of Aβ40 in hippocampus of their offspring,which might cause learning and memory damage of filial mice.

关 键 词：铅 神经毒性 免疫组织化学染色法 β淀粉样蛋白40 

分 类 号：R994.6[医药卫生—毒理学] Q593.2[医药卫生—药学]