赤芍二基源药材的HPLC指纹图谱鉴别及质量评价  

Species Differentiation and Quality Assessment of Radix Paeoniae Rubra from Paeonia lactiflora and Paeonia veitchii by Means of HPLC Fingerprint

作　　者：向楚兵[1] 刘友平[1] 陈鸿平[1] 陈林[1] 杨昌林[1] 

机构地区：[1]成都中医药大学药学院,教育部中药材标准化重点实验室,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137

出　　处：《中国实验方剂学杂志》2011年第23期43-48,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中医药行业科研专项项目(200707007);质检公益性行业科研专项(2007GYB215)

摘　　要：目的:通过HPLC指纹图谱研究,快速有效鉴别北赤芍与川赤芍,并评价其质量。方法:采用高效液相色谱法,分别以北赤芍与川赤芍对照药材为参照图谱,对不同产地、不同采收期的12批北赤芍与29批川赤芍进行指纹图谱研究,同时对其进行相似度评价,并采用判别分析,建立北赤芍与川赤芍的Fisher线性判别函数。结果:北赤芍对照药材与川赤芍对照药材的相似度约为0.7,<0.9,12批北赤芍与29批川赤芍与其相应对照药材的相似度都在0.9以上;北赤芍与川赤芍的Fisher线性判别函数为:Y北赤芍=139.797X1+21.961X2+30.927X3-14.796,Y川赤芍=45.688X1-10.867X2+69.898X3-13.440(自变量X1,X2,X3分别表示以芍药苷为参照峰的儿茶素、芍药内酯苷及苯甲酸的相对峰面积)。结论:采用HPLC指纹图谱,结合相似度评价或判别分析,可快速、有效鉴别北赤芍与川赤芍。川赤芍与北赤芍的化学成分既有共性,又存在差异,且川赤芍更复杂。物种来源不同是导致二者化学成分存在差异的主要因素,而产地及采收期对其影响不大,故应当根据上述研究并结合相关实验,制定规范的赤芍二基原药材质量标准,指导临床安全有效用药。Objective：To quickly and effectively discriminate Radix Paeoniae Rubra originates from Paeonia lactiflora and Paeonia veitchii,and evaluate the quality of them by HPLC fingerprint.Method：HPLC fingerprint was adopted to analyze 12 batches of P.lactiflora and 29 batches of P.veitchii that from different fields and picking time,and setting the profile of reference crude drug of P.lactiflora and P.veitchii Lynch as a control.The similarity was evaluated according to the software-＇Herb Fingerprint Similarity Assessment System＇（2004A）,and established Fisher＇s linear discriminant functions were astablished by discriminant analysis.Result： The similarity index of reference crude drug of P.lactiflora and P.veitchii is about 0.7,lower than 0.9.The similarity index of 12 batches P.lactiflora and 29 batches P.veitchii with their relative reference crude drug is both above 0.9.The Fisher＇s linear discriminant functions was as follows： Y（P.lactiflora）=139.797X1＋21.961X2＋30.927X3-14.796,Y（P.veitchii）=45.688 X1-10.867X2＋69.898X3-13.440（The independentsX1,X2,X3were respectively stand for relative area of catechin,albiflorin and benzoic acid with paeoniflorin area as reference）.Conclusion：Combining the chemometric.Such as similarity evalution or discriminant analysis,we could quickly and effectively discriminate Radix Paeoniae Rubra by HPLC fingerprint.Component similarity and differentiation were existing between P.lactiflora and P.veitchii,and P.veitchii was more complex.Species differentiation was the major factor of different constituents between them,fields and picking time were not important.In order to instruct application in clinic safely,it was worth establishing normative quality standard of Radix Paeoniae Rubra by relative research and experiment.

关 键 词：赤芍 高效液相色谱 指纹图谱 鉴别 质量评价 

分 类 号：R284.1[医药卫生—中药学]