HPLC法同时测定利胆排石片中大黄素、大黄酚及大黄素甲醚  

Simultaneous Determination of Emodin,chrysophanol and Physcion In Lidanpaishi Tablet by HPLC

作　　者：李鑫健[1] 方翠芬[2] 祝明[2] 

机构地区：[1]浙江中医药大学,杭州310053 [2]浙江省食品药品检验所,杭州310004

出　　处：《中国实验方剂学杂志》2011年第23期82-84,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立高效液相色谱法同时测定利胆排石片中大黄素、大黄酚及大黄素甲醚含量的方法。方法:采用高效液相色谱法,Agilent SB-C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-甲醇-0.1%磷酸(42∶23∶35)为流动相,流速1.0 mL.min-1,检测波长254 nm。结果:大黄素、大黄酚和大黄素甲醚分别在3.4×10-3～1.14μg,5.3×10-3～1.06μg,1.9×10-3～0.49μg线性关系良好,r分别为0.999 9,0.999 9,0.999 6;平均加样回收率分别为101.1%,100.5%,99.3%,RSD(n=6)分别为1.1%,0.9%,1.1%。结论:方法简便快捷,结果准确、可靠,重复性好,可作为利胆排石片质量控制方法之一。Objective：To establish an HPLC method for the content determination of emodin,chrysophanol and physcion in Lidanpaishi tablet.Method：The analysis was carried out with Agilent SB-C18（4.6 mm×250 mm,5 μm）column and the mobile phase consisted of Acetontrile-Methanal-0.1%phosphoric acid solution（42∶35∶23）at a flow of 1.0 mL·min-1.The detection wavelength was 254 nm.Result：The calibration curve was linear within the range of 3.4×10-3-1.14 μg（r=0.999 9）,5.3×10-3-1.06 μg（r=0.999 8） and 1.9×10-3-0.49 μg（r=0.999 2） for emodin,chrysophanol and physcion,respectively.The average recoveries were 101.1%（RSD 1.1%）,100.5%（RSD 0.9%） and 99.3%（RSD 1.1%） for emodin,chrysophanol and physcion,respectively.Conclusion：The method is simple,accurate,reproducible and prcise,can be used for the quality of Lidanpaishi tablets.

关 键 词：利胆排石片 大黄素 大黄酚 大黄素甲醚 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]