牡丹皮等10种中药对白色念珠菌浮游菌和生物膜作用的研究  

Effect of Ten Traditional Chinese Medicine on Planktonic and Biofilm Candida Albicans in vitro

作　　者：宫毓静[1] 刘红[1] 冯淑怡[1] 周艳华[1] 孙明杰[1] 

机构地区：[1]中国中医科学院医学实验中心,北京100700

出　　处：《中国实验方剂学杂志》2011年第23期129-132,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家中医药管理局中医药留学回国人员科技活动择优课题(2007LHR04)

摘　　要：目的:观察牡丹皮等10种中药和土槿皮乙酸体外对白色念珠菌浮游菌和生物膜的作用。方法:10种中药为牡丹皮,黄连,丁香,肉桂,高良姜,桂枝,知母,苦参,蛇床子,白花蛇舌草,用水煎煮后浓缩,提取物用DMSO溶解,培养基稀释,体外微量稀释法检测药物对白色念珠菌浮游菌最低抑菌浓度(MIC)和抑制生物膜50%的浓度(SMIC50)的作用。结果:土槿皮乙酸对白色念珠菌的MIC为15.6 mg.L-1,对生物膜的SMIC50是31.2 mg.L-1。牡丹皮和高良姜对白色念珠菌生物膜有一定的抑制作用,SMIC50分别125,250 mg.L-1。黄连,丁香,肉桂,桂枝,知母,苦参,蛇床子,白花蛇舌草在2 000 mg.L-1均没有表现出抑菌活性。结论:体外微量稀释法检测到土槿皮乙酸对白色念珠菌浮游菌和生物膜具有明显的抑制作用,10种中药材中仅牡丹皮和高良姜的水煎液对白色念珠菌生物膜有一定的抑制作用,其他8种中药材的水煎液对白色念珠菌浮游菌和生物膜的抑制作用不明显。对中药抗真菌活性的评价值得进一步探讨。Objective：To observe the effect of ten traditional Chinese medicine and pseudolaric acid B（PB） on Candida albicans yeast and biofilm in vitro.Method：Moutan Cortex,Coptidis Rhizoma,Caryophylli Flos,Cinnamomi cortex,Alpiniae Officinarum Rhizoma,Cinnamomi Rhizoma Anemarrhenae Rhizoma,Sophore flavescentis Radir,Cnidii Fructus and Hedyotis diffusa Willd.were extracted with water,dissolved in DMSO.The microtiter method was used to test the antifungal effect against planktonic and biofilm C.albicans in vitro.Result： The MIC of PB inhibit the planktonic C.albicans was 15.6 mg·L-1,The MIC50 of PB inhibit the biofilm C.albicans was 31.2 mg·L-1.The MIC50 of Moutan Cortex and Alpiniae Officinarum Rhizoma inhibit the biofilm C.albicans was 125 mg·L-1 and 250 mg·L-1,respectively.The other medicines did not show any antifungal effect at 2 000 mg·L-1.Conclusion： PB showed some antifungal effect against planktonic and biofilm C.albicans,all the other traditional Chinese medicine water preparations did not show much activity against planktonic and biofilm C.albicans.The water extracts of traditional Chinese medicine did not showed much antifungal activity against the planktonic and biofilm C.albicans.The evaluation of the antifungal activity of traditional Chinese medicine need to be further discussed.

关 键 词：抗真菌中药 白色念珠菌 生物膜 

分 类 号：R285.5[医药卫生—中药学]