肉桂水提液对全脑缺血再灌注损伤大鼠MAO和CAT的影响  

The Effects of Aqueous Extract of Cinnamon cassia on MAO and CAT in the Cerebral Ischemia Reperfusion Injury Rats

作　　者：黄宏妙[1] 郭占京[1] 蒋凌风[1] 罗佩卓[1] 梁晓艳[1] 

机构地区：[1]广西中医学院药学院,南宁530001

出　　处：《中国实验方剂学杂志》2011年第23期159-161,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西壮族自治区教育厅科研项目(200911LX209)

摘　　要：目的:研究肉桂水提液对大鼠全脑缺血再灌注损伤的保护作用。方法:健康SD雄性大鼠50只随机平均分5组:正常组、模型组和肉桂水提液低、中、高剂量给药组(10,20,40 g.kg-1)。采用4-血管阻断(4-VO)方法建立全脑缺血再灌注动物模型,造模前ig给药7 d,造模后3 h取材,利用分光光度法检测大鼠心、肝、脑、肾等组织中单胺氧化酶(MAO)和过氧化氢酶(CAT)的活力。结果:与模型组比较,肉桂水提液各剂量组均能显著降低各组织中MAO(P<0.05或P<0.01)的活力,并以低剂量组效果最佳;高剂量给药组能显著提高心、肝、脑、肾各组织中CAT活力(P<0.05或P<0.01);低剂量给药组和中剂量给药组对提高心、脑组织中的CAT活力具有一定的显著性差异(P<0.01或P<0.05),但对提高肝、肾组织中的CAT活力没有显著性差异。结论:肉桂水提液对脑缺血再灌注损伤有保护作用,其机制可能与抗脂质过氧化和抑制单胺氧化酶活力有关。Objective：To study protective effects of aqueous extract of Cinnamon cassia on the whole cerebral ischemia reperfusion injury in rats.Method： Fifty SD male rats were randomly divided into five groups： normal group,model group,C.cassia group（10,20,40 g·kg-1 as low,moderate and high dose respectively）.The model of the whole cerebral ischemia reperfusion injury was established by four-vessel occlusion（4-VO）.Intragastric administration of C.cassia continued 7 days before modeling and stoped the administration 3 h after the modeling.The activities of MAO and CAT in the brain,heart,liver,kidney tissues were determined by the Ultraviolet Spectrophotometer.Result： Compared with the model groups,the activity of MAO was decreased remarkably（P0.05 or P0.01） in all tissues by all doses of C.cassia and the result of the low dose was the best.The activity of CAT in all tissues was enhanced remarkably（P0.05 or P0.01） by the high dose,while the activity of CAT in brain and heart was enhanced remarkably（P0.05 or P0.01） by the low group and moderate group,without effects in liver and kidney.Conclusion： Aqueous extract of C.cassia had protective effects on the whole cerebral ischemia reperfusion injury in rats,and the mechanism may relate to lipid peroxidation and inhibitory action of MAO.

关 键 词：肉桂 脑缺血再灌注 单胺氧化酶 过氧化氢酶 

分 类 号：R285.5[医药卫生—中药学]