荷叶铁线蕨水提液对人胚肝细胞增殖和功能的影响  

The Proliferation and Function in Human Embryonic Hepatocytes Treated by Adiantum reniforme Extracts

作　　者：李国利[1] 陈洪源[1] 孙厚良[1] 胡艳玲[1] 张俊磊[2] 刘佳[2] 

机构地区：[1]重庆三峡医药高等专科学校,重庆404120 [2]第三军医大学,重庆400030

出　　处：《中国实验方剂学杂志》2011年第23期165-167,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：重庆市教委科学技术课题(KJ092102)

摘　　要：目的:研究荷叶铁线蕨对人胚肝细胞增殖和功能的影响。方法:人胚肝细胞L-O2 96孔板104/孔培养,经质量浓度为1 g.mL-1荷叶铁线蕨水提液梯度稀释后处理24 h,采用MTT法检测细胞增殖情况;溴甲酚绿比色法检测白蛋白分泌水平;酶标仪法测定乳酸脱氢酶(LDH)活力。结果:荷叶铁线蕨水提液可以促进人胚肝细胞增殖,且具有剂量依赖性;在药物质量浓度为100 g.L-1时细胞白蛋白分泌量达到最高2.14 g.L-1,并显著降低了LDH的活力(P<0.05)。结论:荷叶铁线蕨水提液可以促进人胚肝细胞增殖,增强细胞保肝降酶作用。Objective：To study the proliferation and function in human embryonic hepatocytes treated by Adiantum reniforme L.var.sinense Y.X.Lin extracts.Method： Human embryonic hepatocytes L-O2 104in culture were treated for 24 h with 1 g·mL-1 the aqueous extract solution.The cell proliferation was measured by the MTT assay.The albumin secretion was detected by the bromcresol green colorimetry.The activity of lactate dehydrogenase（LDH） in culturalmedia were measured by enzyme linked immunosorbent assay.Result： The extracts promoted the cell proliferation with dose-dependent.At drug concentration in 100 g·L-1,the aqueous extract solution increase the secretion of albumin to 2.14 g·L-1,and decreased LDH activity.Conclusion： The extract of A.reniforme can promote the hepatic proliferation and function.

关 键 词：荷叶铁线蕨 人胚肝细胞 细胞增殖 肝功能 

分 类 号：R285.5[医药卫生—中药学]