壳聚糖护肤液促进大鼠伤口愈合的研究  

Chitosan liquid improves wound healing in rats

作　　者：杨虎[1] 郑丽君[1] 黄新建[1] 张梦媛[1] 谈伟强[1] 

机构地区：[1]浙江大学医学院附属第一医院,杭州310003

出　　处：《中华皮肤科杂志》2011年第12期891-893,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金（30800228）;浙江省医药卫生科学研究基金（2009A058）;浙江省公益技术研究社会发展项目（2010C33170）

摘　　要：目的探讨壳聚糖护肤液促进伤口愈合的作用。方法在大鼠背部伤口模型上用药，对壳聚糖护肤液组（A组）、碱性成纤维细胞生长因子组（B组）、生理氯化钠溶液组（c组）进行两两对照，肉眼观察伤口愈合时间，并于伤后3、7、14d，取皮肤修复组织行光镜观察。结果A组愈合时间为（17-3±1．35）d，B组为（18．2±1．15）d，C组为（24．0±1．37）d。A组与B组的愈合时间差异无统计学意义（P〉0．05），A组、B组与c组的愈合时间差异有统计学意义（P〈0．05）；壳聚糖护肤液在伤121愈合的早期促进毛细血管芽和血管内皮细胞生成，中期促进成纤维细胞和胶原纤维生成，后期使胶原纤维排列有序。结论壳聚糖护肤液具有较好的促进伤口愈合的作用。Objective To study the promotive effect of chitosan liquid on wound healing. Methods Wound model was established on the back of 60 rats, which were classified into 3 groups to be treated with topical chitosan liquid （group A）, basic fibroblast growth factor （group B）, and sodium chloride physiological solution （group C）, respectively. The time required for the healing of wound was recorded. Regenerated tissues were resected from the rats on day 3, 7 and 14 after the establishment of wound model, and observed with light microscopy. Results The time required for the healing of wound was 17.3 ＋ 1.35 days in group A, 18.2 ＋ 1.15 days in group B, and 24.0 _＋ 1.37 days in group C. For the time required for the healing of wound, no significant difference was observed between the group A and B （P 〉 0.05）, but group C significantly differed from group A and B （both P 〈 0.05）. Chitosan liquid accelerated the generation of capillary sprouts and vascular endothelial cells at the early stage of wound healing, promoted the production of fibroblasts and collagen fibers at the middle stage, and improved the organization of collagen fibers at the late stage. Conclusion Chitosan liquid exerts a promising promotive effect on wound healing.

关 键 词：促进伤口愈合 护肤液 壳聚糖 碱性成纤维细胞生长因子 大鼠 伤口愈合时间 时间差异 胶原纤维 

分 类 号：R965[医药卫生—药理学]