广东截叶胡枝子根瘤菌株系的发现及其16S rDNA分析  

Discovery and identification of rhizobia associated with Lespedeza cuneata in Guangdong

作　　者：刘明骞[1] 丁美美[1] 欧阳昆唏[2] 孙佩光[2] 周玮[1] 骈瑞琪[1] 陈晓阳[1] 

机构地区：[1]华南农业大学林学院,广东广州510642 [2]北京林业大学生物科学与技术学院,北京100083

出　　处：《广东农业科学》2011年第22期138-140,共3页Guangdong Agricultural Sciences

基　　金：省部产学研项目(2006D90204004);广州市科技计划项目(2010Z1-E241);农业部948项目(2011-Z50)

摘　　要：从截叶胡枝子的根瘤中分离出30个分离物,经纯化、镜检后得到26株待测菌株。将其16S rDNA部分序列测序后用DNAMAN和MAGE 5.0软件进行分析,并与参比菌株相比较。结果表明,去除重复后共获得18株根瘤菌菌株,且可将其分为两大类,分别属于慢生根瘤菌属(Bradyrhizobium)和中华根瘤菌属(Sinorhizobium)。26 tested strains were isolated and purified in 30 isolates which were isolated from Lespedeza cuneata. The partial sequence of its 16S rDNA sequences were tested, and the software DNAMAN and MAGE 5.0 were used to analysis these sequences. The results showed that 18 rhizobium strains were identified and belonged to Braclyrhizobium and Sinorhizobium compared with the known strains.

关 键 词：截叶胡枝子 根瘤菌 16S RDNA 

分 类 号：Q939.114[生物学—微生物学]