纳米二氧化锰对大鼠腹侧中脑的损伤作用  

The injury effect of nano-MnO_2 on the ventral midbrain of rats

作　　者：史婷婷[1] 李涛[1] 晋光荣[1] 王健华[1] 刘袁静[1] 陈哲[1] 朱建宝[1] 

机构地区：[1]东南大学医学院人体解剖与组织胚胎学系,南京210009

出　　处：《神经解剖学杂志》2011年第6期623-627,共5页Chinese Journal of Neuroanatomy

基　　金：环境医学工程教育部重点实验室开放课题基金(2010EME008)

摘　　要：目的:探讨纳米二氧化锰(nano-MnO2)对大鼠腹侧中脑的损伤作用。方法:大鼠在脑立体定位下,实验组脑内注射nano-MnO2,对照组脑内注射生理盐水(NS),各组分别于注射1周后用免疫组织化学方法检测酪氨酸羟化酶(tyrosine hydroxylase,TH)、胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)及诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)的表达变化。结果:免疫组织化学染色结果显示,实验组大鼠注射1周后,腹侧中脑损毁侧与对侧相比,TH阳性细胞明显减少(P<0.05),GFAP及iNOS阳性细胞明显增多(P<0.05);对照组大鼠注射1周后,腹侧中脑损毁侧与对侧相比,TH、GFAP和iNOS免疫反应阳性细胞均无明显变化(P>0.05)。结论:脑内注射nano-MnO2能引起大鼠中脑多巴胺能神经元的破坏,GFAP和iNOS的表达增加。Objective： To observe the injury effect of nano-MnO2 on the ventral midbrain of rats.Methods： Nano-MnO2 or 0.9% saline was stereotactically injected into the ventral midbrain of nano-MnO2-treated group and saline-treated group rats.After being injured 1week,the expression of tyrosine hydroxylase（TH）,glial fibrillary acidic protein（GFAP） as well as inducible nitric oxide synthase（iNOS）were detected by immunohistochemical staining.Results： After 1 week of injection,there was a significant decrease of TH positive cells in the damaged side compared to the contralateral（P〈0.05）,while the positive cells of GFAP and iNOS was increased（P0.05） among nano-MnO2-treated rats.But among saline-treated rats,there was no significant difference in terms of the numbers of TH,GFAP and iNOS positive cells between the damaged side and the contralateral side（P〉0.05）.Conclusion： Intracerebral injection of nano-MnO2 might cause damage of dopaminergic neurons,increase GFAP and iNOS expression.

关 键 词：纳米二氧化锰 多巴胺能神经元 胶质纤维酸性蛋白 诱导型一氧化氮合酶 

分 类 号：R114[医药卫生—卫生毒理学]