邻菲啰啉-Fe(Ⅱ)光度法间接测定盐酸吡硫醇  

Spectrophotometric indirect determination of pyritinol hydrochloride by o-phenanthrolinehydrate-Iron(Ⅱ)

作　　者：过治军[1,2] 石灵[1] 冯素玲[3] 崔凤灵[3] 

机构地区：[1]河南师范大学生命科学学院,新乡453007 [2]河南省高校道地中药材保育及利用工程技术研究中心,新乡453007 [3]河南师范大学化学与环境科学学院,新乡453007

出　　处：《分析试验室》2011年第12期5-7,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(30970696)资助;河南省生物化学与分子生物学重点学科资助

摘　　要：在酸性介质中,盐酸吡硫醇可将Fe(Ⅲ)还原为Fe(Ⅱ),邻菲啰啉能与Fe(Ⅱ)显色,最大吸收波长为508 nm,通过测定Fe(1I)的量间接的测定了盐酸吡硫醇的量。建立了测定盐酸吡硫醇质量浓度的新方法。盐酸吡硫醇在0.010～6.0μg/mL范围内与吸光度呈良好的线性关系,线性回归方程为ΔA=-0.00373+0.15558ρ(μg/mL),线性相关系数为0.9996,检出限为0.004μg/mL。方法已用于实际药品中盐酸吡硫醇含量的测定。A simple and sensitive spectrophotometric method is described for the determination of pyritinol hydrochloride.The method is based on that pyritinol hydrochloride can be oxidized by Fe（Ⅲ） in acidic solution.Fe（Ⅱ） can form a complex with o-phenanthroline hydrate,of which the maximum absorption wavelength is at 508 nm.Linearity is obtained in the range of 0.010～6.0 μg/mL with a detection limit of 0.004 μg/mL.The linear regression equation isΔA=-0.00373＋ 0.15558ρ（μg/mL）,with a linear correlation of 0.9996.The proposed method was used to determine pyritinol hydrochloride in pharmaceuticals.The results were compared with those obtained by standard method with satisfaction.

关 键 词：盐酸吡硫醇 Fe(Ⅲ) 邻菲啰啉 分光光度法 

分 类 号：O657.3[理学—分析化学]