聚次甲基蓝修饰玻碳电极测定叶酸  

Determination of folic acid by a poly(methylene blue)-modified glassy carbon electrode

作　　者：古宁宇[1] 刘彩胜[1] 

机构地区：[1]南昌大学化学系,南昌330031

出　　处：《分析试验室》2011年第12期26-30,共5页Chinese Journal of Analysis Laboratory

基　　金：江西省科技支撑项目(2010BGB01001)资助

摘　　要：利用循环伏安法将次甲基蓝修饰到玻碳电极表面,制备了聚次甲蓝修饰电极(PMB/GCE),并研究了此电极的化学性质及对叶酸的电化学响应特性。在磷酸盐缓冲液中PMB/GCE电极对叶酸有良好的催化作用并出现一个灵敏的还原峰,用循环伏安法测得峰电流与叶酸的浓度在0.1×10-3～2.9×10-3mol/L范围内呈良好线性关系,方法检出限(S/N=3)为0.575×10-6mol/L。并用此法对叶酸药片进行了测定和加标回收试验RSD<5%。A poly（methylene blue） modified glassy carbon electrode（PMB/ GCE） was prepared by cyclic voltammetry.Electrochemical properties of PMB/ GCE electrode and response characteristics of folic acid on the PMB/ GCE were investigated.The results showed that the PMB/ GCE modified electrode exhibited effective electrocatalysis for reduction of folic acid in PBS solution.A sensitive reduction peak for folic acid was observed in the voltammogram.The reduction peak current was linear with the concentration of folic acid over the range from 1.0×10-4 to 2.9×10-3mol/L.The detection limit of the method（S/N=3）was 5.75×10-7mol/L.The proposed method was used for the determination of folic acid in tablets and recovery of folic acid samples.The results were consistent with the marked values and RSD was less than 5%.

关 键 词：次甲基蓝 叶酸 循环伏安法 

分 类 号：O657.1[理学—分析化学]