高效液相色谱法测定化妆品中的7种色素  

Determination of 7 colorants in cosmetics by high performance liquid chromatography

作　　者：李玮[1] 芮昶[1] 屠海云 赵凯[1] 王啸[1] 周敏[1] 王娜[1] 

机构地区：[1]杭州市质量技术监督检测院,杭州310019

出　　处：《分析试验室》2011年第12期85-89,共5页Chinese Journal of Analysis Laboratory

摘　　要：建立了用高效液相色谱-二极管阵列检测法(HPLC-DAD)同时检测化妆品中7种着色剂(胭脂红Ponceau)、萘酚黄(Naphthol Yellow S Hydrate)、日落黄(Food Yellow 3)、酸性红33(Acid Red 33)、诱惑红(Allura Red AC)、酸性红13(Acid Red 13)、酸性橙(Orange II))的检测方法。不同种类的化妆品采用不同的样品前处理方法提取后,用C18色谱柱分离,以甲醇-乙酸胺为流动相进行梯度洗脱,检测波长为240nm,14 min内可对7种目标物同时进行检测,且各化合物都达到基线分离。经测定,该方法的平均回收率为90.5%～100.6%,相对标准偏差为3.2%～6.7%,方法检出限萘酚黄为5 mg/L,其余为3 mg/L。方法简单、快速,能有效地提取、分离和测定化妆品中7种着色剂。方法可用于实际化妆品的检测。Ananalytical method based on high performance liquid chromatography with diode array detector（HPLC-DAD） has been established for the simultaneous determination of 7colorants（Ponceau,Naphthol Yellow S Hydrate,Food Yellow 3,Acid Red 33,Allura Red AC,Acid Red 13,Orange II） in cosmetics.The different kinds of samples were treated with different preparation methods.The obtained liquid samples were analyzed by HPLC using a Diamonsil C18 column（250 mm×4.6 mm×5μm）.Methanol/ ammonium acetate（CH3COO NH4） was used as the mobilephase for gradientelution.The wave length of detection is 240nm.The 7 colorants were separated with in 14 min.The average recoveries were from 90.5% to 100.6% with the relative standard deviations（RSDs） between 3.2% and 6.7%.The limits of detection（LOD） were 3 mg/L except that Naphthol Yellow S Hydrate was 5 mg/L.The method is simple,rapid,accurate and suitable for the determination of 7 colorants.

关 键 词：高效液相色谱 色素 化妆品 

分 类 号：O657.7[理学—分析化学]