农杆菌介导的抗病基因对番茄的遗传转化  

Agrobacterium-mediated introduction of disease-resistant genes into tomato(Lycopersicon esculentum Mill)

作　　者：于惠敏[1] 石竹[1] 邵洪伟[1] 李玮[1] 侯丙凯[2] 

机构地区：[1]齐鲁师范学院生物系,山东济南250013 [2]山东大学植物细胞工程与种质创新教育部重点实验室,山东济南250100

出　　处：《山东大学学报（理学版）》2011年第11期1-4,7,共5页Journal of Shandong University(Natural Science)

基　　金：山东省教育厅科技计划项目(H02I51)

摘　　要：通过农杆菌介导法将含有几丁质酶基因和β-1,3-葡聚糖酶基因的双价植物表达载体pBLGC转入番茄子叶外植体。经过共培养、卡那霉素筛选和分化再生,获得了23株卡那霉素抗性植株。PCR检测和Southern Blot检测结果表明,有7株呈阳性检测反应。通过病原真菌接种试验证明,有3株转基因植株表现出较强的抗病性反应。实验结果为进一步研究番茄抗病性和培育抗病番茄新品种奠定了重要基础。A plant expression vector pBLGC containing two disease-resistant genes,the Chitinase gene and the Glutanase gene,was introduced into cotyledon explants of tomato via Agrobacterium-mediated genetic transformation.After co-culture,kanamycin selection and regeneration,23 kanamycin-resistant plants were obtained.Among these,7 plants were demonstrated to be transgenics by PCR and PCR-Southern Blot analysis.Through pathogenic fungus inoculation,it was shown that 3 transgenic plants had strong disease resistance.These results will lay an important basis for the further study of tomato disease resistance and for tomato genetic improvement.

关 键 词：农杆菌 番茄 几丁质酶 葡聚糖酶 抗病性 遗传转化 

分 类 号：Q943[生物学—植物学]