豌豆种质表型性状SSR标记关联分析  

Association Analysis Between Morphological Traits of Pea and its Polymorphic SSR Markers

作　　者：顾竟[1] 李玲[2] 宗绪晓[1] 王海飞[1] 关建平[1] 杨涛[1] 

机构地区：[1]中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程,北京100081 [2]辽宁省农业科学院经济作物研究所,辽阳111000

出　　处：《植物遗传资源学报》2011年第6期833-839,共7页Journal of Plant Genetic Resources

基　　金：现代农业产业技术体系专项(CARS-09);农业部作物种质资源保护项目(NB2010-2130135-25-09)

摘　　要：关联分析是以连锁不平衡原理为基础,鉴定某一群体内表型性状与遗传标记或候选基因间关系的遗传分析方法。本研究利用59个多态性SSR标记,对192份豌豆种质进行全基因组扫描,以分析SSR位点遗传多样性,寻找其连锁不平衡位点;采用TASSEL软件的一般线性模型,利用59个SSR标记对19个形态性状进行关联分析。结果显示SSR位点间有较高的多态性和一定程度的连锁不平衡,共检测出32个SSR标记位点与14个表型性状相关联,一些SSR标记与2个或多个形态性状相关联。Association analysis is an approach to identify the relationship of molecular markers or candidate genes with morphological traits in a given population based on linkage disequilibrium.59 polymorphic SSR markers were applied for genotyping on 192 core collections of pea（Pisum sativum L.）to identify their genetic diversity,and to detect linkage disequilibrium of pair wise loci in the targeted population composed of the 192 core collections.The association analysis between 59 SSR loci and 19 morphological traits was performed by using TASSEL GLM（General Linear Model）program.High polymorphism and various degree of linkage disequilibrium（LD）were detected among SSR loci.Associations between 32 SSR loci and 14 morphological traits were detected,some loci were found associated with two or more morphological traits.

关 键 词：豌豆 SSR 连锁不平衡 关联分析 

分 类 号：S643.3[农业科学—蔬菜学]