甘蓝型特高含油量油菜种质及其主栽品种的指纹图谱构建  

Fingerprinting Construction of Brassica napus Germplasm with Super-high Oil Content and Main Rapeseed Cultivars

作　　者：赵卫国[1,2] 王灏[1,2] 李殿荣[1,2] 田建华[1,2] 赵亚军[1,2] 李保军[1,2] 王晓东[1,2] 王爱娜[1,2] 

机构地区：[1]陕西省杂交油菜研究中心 [2]国家油料作物改良中心陕西油菜分中心,大荔715105

出　　处：《植物遗传资源学报》2011年第6期904-909,共6页Journal of Plant Genetic Resources

基　　金：国家"863"项目(2011AA10A104);陕西省自然科学基金项目

摘　　要：选择特高含油量资源7份,与中国各油菜主产区具有代表性的主栽品种16份,利用SSR多引物组合法开展指纹图谱构建研究。选择多态丰富、图谱清晰稳定且来自不同连锁群的引物28对,对所有材料进行指纹图谱分析,共获SSR指纹条带302条,其中多态性条带为279条,每引物所获条带6～16条,平均10.79条,平均多态率92.38%,通过指纹图谱将所有材料有效地区别开来。用非加权类平均法(UPGAM)聚类分析显示,高油材料之间以及高油材料与主栽品种之间遗传距离均有较大差异,在遗传距离0.171处可将23份材料分成9个类群,其中7份高油材料分处4个类群,遗传距离差异显著;而其他8份主栽品种被分别聚类在另外5个类群中,主栽区域特别明显;所有材料间皆具有丰富的遗传多样性,其中高油材料与主栽品种间遗传差异更大。In this paper,we selected 7 rapeseed germplasms with super-high oil content and 16 main rapeseed cultivars from different planted regions to construct their fingerprinting by SSR molecular markers.Using 28 SSR primer pairs from different linkage groups,we totally got 302 SSR bands from all materials,279 of which are polymorphism bands,each primer amplified 6-16 bands with 10.79 bands on average,the average of polymorphism rate reach to 92.38%.By this way,we could distinguish all the materials effectively.UPGAM analyzing revealed that there were large differences among the high oil content germplasms also between the high oil content germplasms and the main cultivars.At genetic distance of 0.171,the 23 B.napus could be divided into nine groups,in which the super-high oil content germplasms were divided into four groups,and 8 main cultivars were divided into five groups.All the materials had the most abundant genetic diversity,especially there were large genetic differences between the super-high oil germplasms and the main rapeseed cultivars.

关 键 词：甘蓝型油菜 特高含油量 SSR 指纹图谱构建 

分 类 号：S565.4[农业科学—作物学]