代表性大豆种质异黄酮主要组分含量鉴定  

Major Isoflavone Components in Soybean Seeds from the Representative Core Samples

作　　者：葛一楠[1] 孙君明[1] 韩粉霞[1] 于福宽[1] 张晶莹[1] 马磊[1] 张金巍[1] 闫淑荣[1] 杨华[1] 刘章雄[1] 邱丽娟[1] 

机构地区：[1]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程/农业部北京大豆生物学重点实验室,北京100081

出　　处：《植物遗传资源学报》2011年第6期921-927,共7页Journal of Plant Genetic Resources

基　　金：转基因重大专项(2011ZX08004-003);国家"863"计划(2006AA100104);国家支撑计划(2006BAD13B05和2006BAD01A04);院所基金;国家自然科学基金项目(31171576)

摘　　要：采用HPLC检测方法,对100份不同来源的代表性大豆种质进行异黄酮含量分析,结果显示大豆种质中主要含有6种异黄酮组分,分别为黄豆苷、黄豆黄苷、染料木苷、丙二酰基黄豆苷、丙二酰基黄豆黄苷和丙二酰基染料木苷,且异黄酮含量在不同生态区间和品种间均存在显著差异,变异丰富;南方产区大豆品种的异黄酮总含量最高,黄淮大豆产区次之,北方大豆产区最低;6种主要异黄酮组分含量的变异系数变化范围为33.44%～52.03%。相关分析表明异黄酮总含量与各主要组分含量之间均呈极显著正相关,与脂肪含量呈极显著负相关。在此基础上,筛选出高异黄酮含量的大豆种质2份,分别为平顶黑豆和PI-567479,低异黄酮含量的大豆种质2份,分别为茶色豆和牡丰1号,可以用于大豆异黄酮育种或遗传研究。Isoflavone contents were analyzed by HPLC methods in 100 representative soybean cultivars from different regions in this study.The results showed that all 100 cultivars contained six of major isoflavone components in soybean seeds,including daidzin,glycitin,genistin,malonyldaidzin,malonylglycitin and malonylgenistin,and there were significant difference on the isoflavone content among ecological regions and varieties,which explained the abundant genetic variation of isoflavone content in the soybean germplasm.The average of total isoflavone content in soybean cultivars from south region was the highest,and that from north region was the lowest.Coefficient of variation for the six major isoflavone components was ranged from 33.44% to 52.03%.Correlation analysis showed that there was significantly positive correlation among the isoflavone components,in which correlation coefficient between total isoflavone content and malonylgenistin was the maximum.A significantly negative correlation was presented between total isoflavone content and oil content.Two soybean cultivars with high isoflavone content（cv.Pingdingheidou,and cv.PI-567479）,and two cultivars with low isoflavone content（cv.Chasedou and cv.Mufeng） were selected for soybean breeding and genetic study on the isoflavone.

关 键 词：大豆 种质 异黄酮 HPLC 

分 类 号：S565.1[农业科学—作物学]