培养基中CuSO_4和Fe盐浓度对小麦胚培养再生效果的影响  

Effects of CuSO_4 and Iron Source on the Tissue Culture of Wheat

作　　者：别晓敏[1] 杜丽璞[1] 徐惠君[1] 叶兴国[1] 

机构地区：[1]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程/农业部作物遗传育种重点开放实验室,北京100081

出　　处：《植物遗传资源学报》2011年第6期975-981,共7页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(30971776);国家转基因专项(2008ZX08010-004)

摘　　要：以小麦基因型CB037、新春9号和Bobwhite为材料进行成熟胚和幼胚培养,研究了CuSO4和Fe2-EDTA对小麦组织培养再生效果的影响。结果发现,培养基中添加0.1～4.1μmol/L CuSO4对小麦成熟胚组织培养无显著效果,超过6.1μmol/L对愈伤组织有毒害作用;培养基中添加40μmol/L Fe盐对小麦成熟胚组织培养和植株再生具有促进作用;不同小麦基因型其幼胚培养对Fe盐的反应不同,40μmol/L～50μmol/L Fe盐处理对CB037幼胚再生效果最好,20μmol/L Fe盐浓度对新春9号和Bobwhite幼胚再生效果较好,高浓度铁盐抑制作用显著;在相同培养条件下,CB037幼胚再生率最高,新春9号次之,Bob-white最低。To optimize the concentrations of CuSO4 and iron source used in wheat embryo culture,investigation on the influence of Fe and Cu ions in culture media on wheat regeneration was performed using CB037,Xinchun9 and Bobwhite as materials.The results showed that there was no difference from the efficiencies for embryogenic callus induction and green shoot induction when the concentration of CuSO4 was employed from 0.1 to 4.1μmol/L,however,CuSO4 at 6.1μmol /L was toxic for mature embryos of wheat.Regeneration efficiency from the mature embryos of CB037 was obviously improved on the media containing 40μmol /L iron source.The optimal concentration of iron source were 40 to 50μmol /L when the immature embryos of CB037 were used as explants,both of callus differentiation and green shoots induction in this level were higher than those in the level of 20μmol /L（at random）.For the immature embryo culture of Xinchun9 and Bobwhite,the ideal concentration of iron source was 20μmol /L.The sensibility of different genotypes to iron source was discrepant.In average,the order for regeneration potential of immature embryos among the three wheat genotypes used in this study is CB037,Xinchun 9 and Bobwhite.

关 键 词：小麦 组织培养 CUSO4 Fe盐 

分 类 号：S512.1[农业科学—作物学]