湖北恩施渔塘坝富硒碳质泥岩中的微生物多样性初探  

Primarily Investigating the Diversity of Microorganisms in Selenium-rich Carbonaceous Mudstone of Yutangba,Enshi,Hubei

作　　者：雷磊[1,2] 朱建明[1] 王风平[3] 肖湘[3] 秦海波[1,2] 冯新斌[1] 

机构地区：[1]中国科学院地球化学研究所,环境地球化学国家重点实验室,贵阳550002 [2]中国科学院研究生院,北京100049 [3]国家海洋局第三海洋研究所,福建厦门361005

出　　处：《地球与环境》2011年第4期517-522,共6页Earth and Environment

基　　金：中国科学院知识创新工程重要方向项目(KZCX2-YW-JC101);国家自然科学基金创新群体项目(40721002);国家自然科学基金项目(40973085)

摘　　要：本文以湖北恩施渔塘坝富硒碳质泥岩为研究对象,采用构建16SrRNA基因克隆文库方法首次对其微生物群落结构进行了初步调查,结果发现其中细菌主要特征群落为变形菌(Proteobacteria)34%、酸杆菌(Acidobacteria)43.6%、放线菌(Ac-tinobacteria)12.9%和未分类细菌9.5%。古菌主要优势菌群为泉古菌(Crenarchaeota)66.67%,未分类古菌32%。表明高硒样品中具有丰富的微生物多样性。该结果有助于进一步理解高硒环境下硒生物地球化学转化过程中微生物的重要作用,并为硒中毒地区的生物修复和恩施硒资源的安全开发提供参考依据。In order to investigate the microbial diversity of the selenium(Se)-rich carbonaceous mudstone of Yutangba,Enshi,Hubei Province,the microbial community structures have been primarily characterized by using restriction fragment length polymorphism(RFLP) and 16S rRNA gene library analyses based on the DNA extracted from the mudstone.Phylogenetic analysis revealed that most of the bacterial clones were affiliated with members of the Acidobacteria family(43.6%),Proteobacteria family(34%),Actinobacteria family(12.9%) and the members of unclassfied bacteria(3%).In the Archaea domain,the dominant Archaea belonged to the members of the Crenarchaeota family(66.7%),others were unclassified(32%).These results showed that there is a rich diversity of microorganisms in Se-rich carbonaceous mudstone,which is helpful to further understand the significant role of microorganisms in the biogeochemical cycle of Se in the Se-high surface environment,and to provide the referred evidence for bio-remediation of Se-polluted areas and safe utilization of mineral resources on Se in Enshi Prefecture,China.

关 键 词：硒 富硒碳质泥岩 微生物多样性 

分 类 号：S154.3[农业科学—土壤学]