益智仁挥发油抗帕金森模型小鼠黑质神经元凋亡的作用研究  

Effects of Volatile Oil Extracted from Alpinia oxyphylla on the Apoptosis of Substantial Nigra Neuron in PD Mice Model

作　　者：黄凌[1] 朱毅[2] 邝少轶[1] 

机构地区：[1]海南医学院药学院药理教研室,海口市571101 [2]海南省药品检验所,海口市570216

出　　处：《中国药房》2011年第47期4430-4433,共4页China Pharmacy

摘　　要：目的:研究益智仁挥发油对帕金森(PD)模型小鼠黑质致密部尼氏小体、酪氨酸羟化酶(TH)表达的影响和神经元凋亡的对抗作用。方法:实验分为6组,即正常对照(等容生理盐水)、模型(等容生理盐水,ig生理盐水第6天起同时复制模型,连续7d,每天1次)、司来吉兰(10mg·kg-1,ig司来吉兰第4天起同时复制模型,连续7d,每天1次)和益智仁挥发油高、中、低剂量(2.5、0.833、0.278mL·kg-1,ig益智仁挥发油第6天起同时复制模型,连续7d,每天1次)组。ip1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)复制C57BL小鼠PD模型。采用尼氏染色法观察尼氏小体表达;SABC免疫组化染色法观察益智仁挥发油对TH表达的影响;TUNEL染色法检测黑质神经元凋亡。结果:与正常对照组比较,模型组小鼠黑质神经元的尼氏小体、TH表达阳性细胞数明显减少,神经元凋亡显著增加;与模型组比较,益智仁挥发油高、中剂量组小鼠黑质神经元内尼氏小体显著增多;益知仁挥发油高、中、低剂量组TH表达阳性细胞数显著增多,而凋亡细胞数量显著减少。结论:益智仁挥发油能对抗PD模型小鼠黑质神经元细胞凋亡。OBJECTIVE： To study the effect of volatile oil extracted from Alpinia oxyphylla on the expression of Nissl＇ s cyto- ryctes and TH and the apoptosis of substantial nigra neuron in Parkinson＇ s disease （PD） mice model. METHODS： Model mice were divided into 6 groups, i.e. normal control group （constant volume normal saline）, model group （constant volume normal sa- line, model induced by intragastrical administration of normal saline once a day since 6th day for consecutive 7 days）, selegiline group （10 mg.kg1, model induced by intragastrical administration of selegiline once a day since 4th day for consecutive 7 days）, A. oxyphylla volatile oil high-dose, medium-dose and low-dose groups （2.5, 0.833, 0.278 mL-kg-1,model induced by intragastrical administration ofA. oxyphylla volatile oil once a day since 6th day for consecutive 7 days）. MPTP was used to establish C57BL PD mice model. Nissl staining method was used to detect Nissl cytoryctes expression; SABC staining method was used to detect TH expression; TUNEL staining method was used to detect the apoptosis of substantial nigra neuron in mice. RESULTS： Compared with normal control group, Nissl＇ s cytoryctes and TH expression of substantia nigra neurons in model group were significantly reduced, and the apoptosis of substantia nigra neurons in model mice were significantly increased; compared with model group, Nissl＇s cyto- ryctes of PD mice in A. oxyphylla volatile oil high-dose and medium-dose groups increased significantly;TH expression of PD mice in A. oxyphylla voletileoil high-dose, medium-dose, low-dose groups increased significantly, while the apoptosis of substantia nigra neurons were significantly reduced. CONCLUSION： It is suggested that A. oxyphylla volatile oil can relieve the apoptosis of substantia nigra neurons in PD mice.

关 键 词：益智仁 帕金森病 抗凋亡 

分 类 号：R285[医药卫生—中药学] R97[医药卫生—中医学]