高温胁迫对不同种源希蒙得木叶片生理特性的影响  

Effects of high-temperature stress on physiological characteristics of leaves of Simmondsia Chinensis seedlings from different provenances

作　　者：黄溦溦[1] 张念念[1] 胡庭兴[1] 李晓清[2] 何远洋[1] 尹丽[1] 

机构地区：[1]四川农业大学林学院,四川省林业生态工程重点实验室,雅安625014 [2]四川省林业科学研究院,成都610081

出　　处：《生态学报》2011年第23期7047-7055,共9页Acta Ecologica Sinica

基　　金：国家林业局“948”引进项目(20000404);四川省青年基金项目(09ZQ026-057);四川省重点学科建设项目(SZD2004,SZD2008)

摘　　要：利用人工气候室模拟高温环境,研究了不同程度高温处理对3个不同种源(Z1:会东可河;Z2:澳大利亚肯多伯冷;Z3:美国菲尼克斯)希蒙得木叶片相对含水量(LRWC)、叶片光合特性、渗透调节、抗氧化保护酶、膜脂过氧化的影响。结果表明,3个种源希蒙得木幼苗LRWC、净光合速率(Pn)和蒸腾速率(Tr)均随高温胁迫强度的加剧呈极显著下降趋势,叶绿素含量(Chl)、可溶性糖含量显著降低;脯氨酸(Pro)含量呈极显著上升趋势;丙二醛(MDA)含量和相对电导率(REC)均随高温胁迫强度的加剧而显著增加;高温胁迫对希蒙得木幼苗叶片过氧化物酶(POD)活性和超氧化物歧化酶(SOD)活性的影响因种源不同而有所差异,Z1、Z2的POD活性随高温胁迫程度的增加而持续上升,Z3的POD活性呈先上升后降低趋势;Z1、Z3的SOD活性随高温胁迫程度的增加而持续上升,Z2的SOD活性呈先降低后上升趋势。以隶属函数法综合分析各种源希蒙得木幼苗抗高温能力的结果表明,3个种源希蒙得木幼苗的抗高温能力由强到弱依次为:Z1、Z3和Z2。结果表明,长期生长在金沙江干热河谷的Z1可能已经适应当地的生态环境,表现出较好的抵御高温的能力,不同种源耐高温能力的差异可能是通过种源所在地的气候、土壤、海拔等因子综合作用,经过漫长的系统发育,产生不同的变异结果。Using phytotron to simulate high temperature environment, the physiological characteristics of leaves of Simmondsia chinensis seedlings from different provenances （ Z1 ： kehe in China, Z2 ： Condobolin in Australia, Z3 ： Phoenix in USA） under different degree of high-temperature stress （CK, T1 , T2） have been studied. The results showed that with the increase of temperature, the leaf relative water content（LRWC）, net photosynthetic rate（Pn）, transpiration rate （Tr）, chlorophyll （Chl） content, and sugar content decreased significantly. While the contents of praline （Pro）, malondialdehyde （MDA）, and relative conductivity （REC） increased significantly, with the enhancement of temperature stress. However, the effects of high-temperature stress on peroxidase （POD） and superoxide dismutase （SOD） activities were not consistent among different provenances. POD activities of Z~ and Z2 increased with the increase of temperature stress intensity, while that of Z3 first increased and then decreased. SOD activities of Z1 and Z3 increased with the increase of temperature stress intensity, however that of Z2 first decreased and then increased. The photosynthesis mechanisms of S. chinensis were different under diverse high-temperature stress. In high-temperature environment （ T1 ） , a part of plant stomata closed to reduce the water loss and maintain normal physiological functions. Extreme heat- enviroment （ T2） broke the systems of ros generation and elimination, and further led ms abundantly accumulating, light oxidation, mda aggravation, plant cell dehydration, chl contents reduction, and the damage of photosynthetic organization. All of these changes brought about by high-temperature stress induced the decrease of Pn of S. chinensis greatly. Comprehensive evaluation of high-temperature resistance of S. chinensis seedlings of three provenances was conducted using fuzzy subordination method. The order of high-temperature resistance ability （from strong to weak） was K

关 键 词：希蒙得木 高温胁迫 种源 光合 生理特性 

分 类 号：S793.9[农业科学—林木遗传育种]