三叶青总黄酮提取工艺研究  

Study on total flavones exuraction from Radix Tetrastigmae

作　　者：楼肖成[1] 郭勇[1] 

机构地区：[1]浙江省东阳玉米研究所,东阳322100

出　　处：《海峡药学》2011年第11期9-12,共4页Strait Pharmaceutical Journal

基　　金：浙江省农科院重点实验室项目;金华市科研项目

摘　　要：目的优选三叶青总黄酮的最佳提取工艺。方法通过回流提取法、超声提取法及微波法提取法的比较,优选三叶青总黄酮最佳提取方法,且通过单因素考察和正交试验,优化最佳工艺条件,用紫外分光光度法测定总黄酮含量。结果最佳提取方法为微波提取法,最佳工艺条件为:料液比1∶25,60%乙醇,微波功率450w,微波时间15min。结论优选得到的提取工艺合理、操作可行、质量可控。OBJECTIVE To screen the optimal extraction process of total flavones from Radix Tetrastigmae.METHODS Compared with reflux extraction,ultrasonic extraction and microwave extraction,to optimize the best extraction method of total flavones,and through the single factor and orthogonal test to optimize the optimum conditions.The quantities of total flavones were determined by spectrophotometry.RESULTS The best extsaction method was microwave extraction,and the best conditions were：liquid ratio 1：25,60% ethanol,microwave power 450w,and microwave time 15min.CONCLUSION The extracting technology optimized in this experiment is reasonable,practical and controllable.

关 键 词：三叶青 总黄酮 提取 正交试验 

分 类 号：R284.2[医药卫生—中药学]