人CD9基因的克隆及原核蛋白表达  

Cloning and Prokaryotic Expression of Human CD9 Gene

作　　者：郑小莉[1] 罗波[1] 张艺[2] 刘佳佳[3] 

机构地区：[1]泸州医学院基础医学院生物化学教研室,四川泸州646000 [2]泸州医学院基础医学院机能实验室,四川泸州646000 [3]泸州医学院基础医学院免疫学教研室,四川泸州646000

出　　处：《重庆理工大学学报（自然科学）》2011年第11期34-38,共5页Journal of Chongqing University of Technology：Natural Science

基　　金：国家自然科学基金资助项目(30371305);四川省卫生厅基金资助项目(100208)

摘　　要：通过RT-PCR技术,对人CD9基因的全长开放阅读框cDNA进行了扩增、克隆、序列测定和分析,再将cDNA插入原核表达载体PET30a(+)后转化大肠杆菌BL21,进而融合表达重组蛋白PET30a(+)/CD9。实验结果表明:RT-PCR方法扩增人CD9 cDNA],获得其全长开放阅读框为690bp,测序结果与NCBI上登录的人CD9基因序列完全一致;成功融合表达出了重组蛋白PET30a(+)/CD9,重组蛋白的分子量为30 KDa。CD9 protein encoded by CD9 gene is a member of the transmembrane 4 superfamily,also known as the tetraspanin family.This protein mediates signal transduction events that play a role in the regulation of cell development,activation,growth and motility.Here the complete open reading frame（ORF） of CD9 gene was cloned by RT-PCR and prokaryotic expression vector of PET30a（＋）/CD9 was constructed.The PET30a（＋）/CD9 protein was expressed and purified.The results showed that the length of the ORF of CD9 was 690bp and the molecular weight of the expressed PET30a（＋）/CD9 protein was 30KD.All the data indicate that the ORF of CD9 gene was cloned and the PET30a（＋）/CD9 protein was expressed successfully.

关 键 词：CD9基因 克隆 原核表达 

分 类 号：R392.11[医药卫生—免疫学]