不同标本处理方法对ELISA检测乳汁HBV-M结果的影响  

Evaluation of Different Treatment Methods on Results of HBV-M Tested Breast Milk with Enzyme-Linked Immunosorbent Assay

作　　者：薛艳[1] 赵江燕[1] 钱厚明[1] 

机构地区：[1]扬州市第一人民医院检验科,江苏扬州225001

出　　处：《临床误诊误治》2011年第11期85-87,共3页Clinical Misdiagnosis & Mistherapy

摘　　要：目的探讨酶联免疫吸附法(ELISA)检测乳汁乙型肝炎病毒标志物(HBV-M)的最佳标本处理方法。方法选择我院乙型肝炎病毒(HBV)携带产妇144例,按血清HBeAg及前S1抗原(PreS1)是否阳性分为HBeAg及PreS1阳性组(16例)、HBeAg阳性组(11例)、PreS1阳性组(5例)、HBeAg及PreS1阴性组(112例)。无菌采集产后2～4 d的初乳,分别采用原始乳汁静置式孵育定性、冷藏后中间层乳汁静置式孵育定性、原始乳汁振动式孵育定性进行标本处理,以ELISA法检测乳汁HBV-M。结果 HBeAg及PreS1阳性组和HBeAg阳性组、PreS1阳性组3种乳汁处理方法检测HBsAg阳性率达到或接近100.0%。采用原始乳汁振动式孵育定性进行乳汁处理检测HBsAg、HBeAg及PreS1的阳性率高于前两种方法,差异有统计学意义(P<0.05)。同一种处理方法检测HBsAg、HBeAg及PreS1的阳性率较接近,抗-HBe及抗-HBc(稀释)的阳性率低于抗-HBc(原倍),差异有统计学意义(P<0.05)。结论在不具备乙肝病毒核酸检测条件的实验室,可应用原始乳汁振动式孵育定性乳汁处理方法检测HBV携带产妇乳汁HBV-M。Objective To explore the best treatment method of breast milk for detecting HBV-M with enzyme-linked immunosorbent assay（ELISA）.Methods According to HEeAg and Pres1 of blood serum,negative or positive,144 HBV-positive parturients were divided to HBeAg-positive and PreS1-positive group（n=16）,HBeAg-positive group（n=11）,PreS1-positive group（n=5）,and HBeAg-negative and PreS1-negative group（n=112）.Colostrums were collected with asepsis from day 2 to day 5 after parturition.All the samples were treated with standing brood qualitation of colostrums,standing brood qualitation of interlayer breast milk after cold preservation,and vibratory brood of colostrums respectively.HBV-M was detected by ELISA.Results In HBeAg-positive and PreS1-positive group,HBeAg-positive group and PreS1-positive group,positive rates of HBsAg treated with the 3 methods were 100.0% or close to 100.0%.Detection rate of HBsAg,HBeAg,and PreS1 with vibratory brood of colostrums were higher than those with the other 2 treatment methods,and the differences were significant（P0.05）.The detection rates of HBsAg,HBeAg,and PreS1 were at the same level with the same method,but the detection rates of anti-HBe and anti-HBc were lower than that of anti-HBc,and the differences were significant（P0.05）. Conclusion HBV in HBV-positive parturient can be detected with vibratory brood,if there is no access to HBV-DNA detection facilities.

关 键 词：肝炎病毒 乙型 血传病原体 乳汁 酶联免疫吸附测定 

分 类 号：R446.6[医药卫生—诊断学]