双歧杆菌对食物过敏治疗作用的实验研究  

The experimental research on the efficacy of bifidobacterium on food allergy

作　　者：李建杰[1] 刘晓宇[1] 杨成彬[1] 陈思[1] 刘志刚[1] 

机构地区：[1]深圳大学过敏反应与免疫学研究所,518060

出　　处：《免疫学杂志》2011年第12期1048-1051,共4页Immunological Journal

基　　金：国家自然科学基金(30871752);深圳市科技计划基础研究重点项目(深发改[2011]168号);深港创新圈项目(CXQ2008026);深圳大学科研创新团队基金(200904)

摘　　要：目的探讨双歧杆菌对食物过敏的治疗作用。方法 4周龄棕色挪威大鼠(Brown-Norway Rat,BN Rat)构建卵清蛋白(ovalbumin,OVA)致敏动物模型,以OVA 1 mg每天连续灌胃6周,同时分别建立PBS对照组和双歧杆菌治疗组;第6周取血测定血清中OVA特异性IgE(OVA-IgE)水平和IgG(OVA-IgG)水平作为模型评测指标;采用张力换能器测定肠道收缩情况,苏木素-伊红染色(Hematoxylin-Eosin stain,HE)及甲苯胺蓝(Toluidine blue Benzen esulfonic acid,TB)染色观察肠组织病理变化和肠粘膜肥大细胞数目和形态变化。结果第6周时实验组OVA-IgE和OVA-IgG含量显著升高,与对照组相比,OVA-IgE存在显著性差异(P<0.05),OVA-IgG存在极显著差异(P<0.01);经益生菌治疗后,OVA-IgE和OVA-IgG含量显著下降,与实验组相比,存在显著性差异(P<0.05)。双歧杆菌治疗组肠组织收缩较OVA组显著降低,存在显著性差异(P<0.05);HE染色和甲苯胺蓝染色可见双歧杆菌治疗组肠粘膜破坏程度、肠粘膜肥大细胞脱颗粒现象较OVA组缓解。结论 OVA致敏模型和双歧杆菌治疗组模型成功建立,双歧杆菌对食物过敏有较好的治疗作用。To study the therapeutic effects of bifidobacterium on food allergy,4-week-old Brown-Norway Rats（BN） were sensitized by feeding ovalbumin 1 mg every day for 6 weeks to establish food allergy animal model,which were then randomly divided into 3 groups： PBS subgroup,OVA subgroup and bifidobacterium treatment subgroup.Sixth week after,blood of the rats were collected for detection serum OVA specificity IgE（OVA-IgE） level and OVA specificity IgG（OVA-IgG） level as allergic judgment standard;the contractility of isolated intestine sections was studied by transducers;the changes of intestinal tissue pathological and the intestinal mucosa mast cells were observed by Hematoxylin-Eosin（HE） stain and Toluidine blue Benzen esulfonic acid（TB） stain,respectively.OVA-IgE and-IgG levels significantly increased on the sixth week,as compared with the control subgroup（P 〈 0.05 and P 〈 0.01,respectively）.After treatment by bifidobacterium,the levels of OVA-IgE and-IgG decreased（P 〈 0.05）,the contractility of intestine tissue reduced significantly（P 〈 0.05）,and the damage of intestinal mucosa alleviated,and the degranulated mast cells decreased statistically,as compared with OVA subgroup.In conclusion,bifidobacterium have well therapeutical effects in food allergy treatment.

关 键 词：双歧杆菌 食物过敏 肠组织 肥大细胞 

分 类 号：R593[医药卫生—内科学]