Fractalkine对脂多糖激活的小胶质细胞炎症相关因子表达的影响  

Effects of fractalkine on the expression of inflammatory substances in LPS-activated microglia cells

作　　者：祝先进[1] 宋艳芳[2] 张秋玉[3] 曹颖平[1] 许伟群[3] 苏东辉[3] 

机构地区：[1]福建医科大学附属协和医院检验科 [2]福建省人民医院检验科 [3]福建医科大学免疫学系,福建福州350004

出　　处：《细胞与分子免疫学杂志》2011年第12期1298-1300,共3页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的:探讨趋化因子Fractalkine对脂多糖(LPS)诱导的小鼠小胶质细胞(N9)激活时所分泌的TNF-α、IL-1β和一氧化氮(NO)表达的影响。方法:用Fractalkine处理经LPS激活的小鼠小胶质细胞24 h,以ELISA法检测细胞培养上清中TNF-α和IL-1β的浓度,以NO试剂盒检测培养上清中NO的浓度。结果:LPS能够激活小胶质细胞,使IL-1β、TNF-α和NO的表达量与对照组相比明显升高;Fractalkine能够降低LPS激活的小胶质细胞IL-1β、TNF-α和NO的表达。结论:Fractalkine可能通过抑制炎症相关因子的产生而在中枢神经系统中发挥神经保护作用。AIM： To investigate the effect of fractalkine on the expression of the tumor necrosis factor-α（TNF-α）, interleukin-1β （IL-1β）, and nitric oxide （NO） in LPS-activated routine microglia ceils line Ng. METHODS： In vitro LPS- activated microglia cells were treated for 24 h in the presence of fractalkine. The level of TNF-a and IL-1β in the culture supematants were measured by enzyme-linked immunosorbent assay （ ELISA）, The level of NO in the culture supernatants were quantitated by the NO test assay. RESULTS： The concentration of TNF-α, IL-1β and NO in the culture supernatants evidently increased in LPS-activated microglia ceils groups and prominently decreased by the fractalkine co-incubated. CONCLUSION： It is thus concluded that fractalkine has neuroprotective functions by inhibiting the expression of inflammatory factor in activated microgila cells.

关 键 词：小胶质细胞 FRACTALKINE IL-1Β TNF-Α 一氧化氮 

分 类 号：R392.12[医药卫生—免疫学]