油酸型急性肺损伤大鼠肺组织中RhoA的表达及对IL-8、IL-10表达的影响  

Expression of RhoA in the lung tissue of acute lung injury rats and the influence of RhoA on the expression of IL-8 and IL-10

作　　者：崔晴晴[1] 冯学斌[1] 王跃嗣[2] 赵磊[1] 

机构地区：[1]滨州医学院儿科教研室,山东滨州256603 [2]滨州医学院干细胞与组织工程研究所,山东烟台264003

出　　处：《细胞与分子免疫学杂志》2011年第12期1308-1311,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30801353)

摘　　要：目的:观察油酸型急性肺损伤(AIL)大鼠肺组织中小G蛋白(RhoA)的表达以及其对IL-8/IL-10表达的影响,探讨RhoA与急性肺损伤的关系。方法:建立大鼠油酸急性肺损伤模型,检测各组大鼠肺湿/干重比,比较肺组织病理学改变;采用聚合酶链反应检测肺组织匀浆RhoA表达,酶联免疫吸附(ELISA)法测定大鼠IL-10/IL-8的变化。结果:肺W/D损伤组在2、6、12、24、48 h均较对照组明显升高(P<0.01),而治疗组在相同时间点较损伤组降低(P<0.05)。治疗组2、6、12、24、48 h血液中IL-10较对照组升高(P<0.05);IL-10较损伤组同时间点明显升高(P<0.05)。损伤组各时间点血清IL-8较对照组明显升高(P<0.01),而干预组较损伤组同时间点显著降低(P<0.01)。损伤组2、6、12、24、48 h大鼠肺组织RhoA较对照组明显升高(P<0.01);干预组与损伤组同时间点比较无显著差异(P>0.05)。结论:油酸型急性肺损伤大鼠肺组织中RhoA表达增加,而RhoA表达增加可致大鼠肺损伤程度增大,RhoA可能通过上调表达IL-8及下调IL-10表达作用而导致肺损伤程度加大。AIM： To investigate the expression of RhoA in the lung tissue of acute lung injury CALl） rats induced by oleic acid （OA） and explore the influence of RhoA on the level of IL-8 and IL-10 in serum. Discussion RhoA and acute lung injury relationship. METHODS： Establish rat oleic acid acute lung injury model, Wet/Dry weights （W/D） were detected. Pathological changes of the lung tissue were examined. The levels of IL-8 and IL-10 in serum were detected by ELISA and the expression of RhoA in homogenate of lung tissue was determined by PCR. RESULTS： W/D and ALl score in ALl group at 6, 12, 24 and 48 h were higher than those in control group （P 〈 0.01 ） and intervention group at the same time point （P 〈 0.05）. The level of IL-10（ng/L） in serum in ALl group at 2, 6, 12, 24 and 48 h was remarkably higher than that in control group （ P 〈 0.01 ）, but was lower compared with intervention group at the same time point （P〈0.01）. The level of IL-8（pg/L） in serum at 2, 6, 12, 24 and 48 h in ALl group were higher than those in control group （P 〈 0.01 ） and intervention group at the same time point（ P 〈 0.01 ）. Compared with that of control group, the expression of RhoA in homogenate of lung tissue in ALl group at？., 6, 12, 24 and 48 h was higher（P〈0.01）, but there was no difference between ALl group and intervention group at the same time point （ P 〉 0.05 ）. CONCLUSION： The expression of RhoA in lung tissue of ALl rats induced by OA is increased. RhoA expression increase that can cause lung damage degree increase in rats. RhoA can aggravate the pathology changes of ALl rats, which might be related to up regulating expression of IL-8 and down regulating expression of IL-10 by RhoA.

关 键 词：急性肺损伤 油酸 IL-10 IL-8 小G蛋白 

分 类 号：R563[医药卫生—呼吸系统]