小鼠nepmucin与CD31在内皮细胞内定位和循环途径的比较  

Comparision of intracellular localization and recycling route of mouse nepmucin and CD31 in endothelial cells

作　　者：彭晓东[1] 魏大鹏[2] 章崇杰[2] 罗志娟[2] 

机构地区：[1]四川大学华西医院实验医学科临床免疫室,四川成都610041 [2]四川大学基础与法医学院免疫学教研室,四川成都610041

出　　处：《细胞与分子免疫学杂志》2011年第12期1315-1318,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30872305)

摘　　要：目的:研究nepmucin和CD31分子在转染内皮细胞内的分布和循环,探讨nepmucin分子的循环机制。方法:采用激光扫描共聚焦荧光显微技术进行循环实验和内化实验,在nepmucin转染细胞(WT-F2)上,用特异性荧光标记抗nep-mucin抗体和抗CD31抗体检测循环和内化的nepmucin和CD31分子。结果:小鼠nepmucin与CD31分子一样地在内皮细胞内发生循环,但速率明显降低;两种分子在细胞内的分布不同,CD31分子主要沿细胞膜分布,而nepmucin则主要分布在胞质深处及少量分布在细胞膜上。结论:小鼠nepmucin与CD31分子在内皮细胞内的分布和循环机制截然不同。AIM： To compare the localization and recycling between nepmucin and CD31 molecules on transfected endothelial cells, and attempted to clarify the recycling mechanisms of nepmucin in endothelial cells. METHODS： Recycling assay and internalization assay were employed to compare the localization and recycling pathway of nepmucin and CD31. The internalized and recycling nepmucin and CD31 molecules on transfected endothelial cells were double or single stained with specific fluorchrome-labeled mono- clonal antibodies against nepmucin （Alexa Fluor 488-ZAQS） and/or CD31 （Alexa Fluor 488-anti-CD31 or Alexa Fluor.594- anti-CD3l ）, then observed under confocal microscopy. RESULTS： Mouse nepmucin underwent intracellular recycling like CD31, but which recycling rate was significantly lower. The CD31 and nepmucin molecules showed largely distinct localization in endothelial cells. CD31 was found mainly on the cell surface, while nepmucin was found predominantly in the deep area of cytoplasm and partly on the cell membrane. CONCLUSION： The distribution of mouse nepmucin in endothelial cells are different from CD31. Nepmucin underwent intracellular recycling like CD31 but employed differ ent mechanisms.

关 键 词：小鼠nepmucin CD31 定位 循环 

分 类 号：R392.11[医药卫生—免疫学]