职业铅接触工人外周血T细胞Vβ基因谱系明显缺失  

Change in the pattern of peripheral blood TCR V β gene repertoire in occupational lead-exposed workers

作　　者：刘思初[1] 李萡[1,2] 牛宇哲[1,2] 刘薇薇[3] 杨志前[3] 陈少华[1] 杨力建[1] 李扬秋[1,2] 

机构地区：[1]暨南大学医学院血液病研究所,广东广州510632 [2]暨南大学医学院教育部再生医学重点实验室,广东广州510632 [3]广州市第十二人民医院,广东广州510620

出　　处：《细胞与分子免疫学杂志》2011年第12期1322-1324,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30972455);中央高校基本科研业务费专项资金项目(21610408);广州市医药卫生科技重点项目(2009-ZBL-18)

摘　　要：目的:分析职业铅接触工人外周血中T细胞受体Vβ基因(TCR Vβ)谱系分布和克隆性特点,进一步了解铅接触后对T细胞免疫的影响。方法:利用RT-PCR分别检测6例职业铅接触工人和6例健康体检者的外周血中各TCR Vβ亚家族的表达情况;阳性产物经荧光素标记和基因扫描分析其CDR3长度以了解T细胞克隆性。结果:所有健康体检者均可检测到24个Vβ亚家族并呈多克隆,而6例职业铅接触工人外周血TCR Vβ亚家族表现明显的限制性利用,仅能检测到1～7个,并且多数所检测到的Vβ亚家族T细胞呈现为寡克隆或双克隆增殖,发生克隆性改变的亚家族主要集中在Vβ1和Vβ16。结论:职业铅接触可能在不同程度上影响机体TCR Vβ谱系的利用和克隆性改变,可能与铅的免疫毒性有关。AIM： To investigate the distribution and clonality of TCR Vβ subfamily in peripheral blood from workers exposed to lead, in order to understand the change in T cell immunity of occupational lead-exposed workers. METHODS： The CDR3 of TCR Vβ 24 subfamily genes was amplified in peripheral blood mononuclear cells （PBMC） from 6 lead-exposed workers and 6 healthy individuals using RT-PCR, the positive PCR products were further subjected by genescan analysis to identify T cell clonality. RESULTS： All 24 TCR Vβ subfamilies were detected in PBMC from 6 healthy individuals which all have polyclonal patterns. Only 1 -7 Vβ subfamilies could be identified in lead-exposed workers. In detected TCR Vβ subfamilies, almost oligoclonal and biclonal patterns which mainly in V[51 and Vβ16. CONCLUSION： The restricted expression and clonal expansion of TCR Vβ subfamily have been found in occupational lead-exposed workers, it may have some relationship with lead toxicity damage to the immune function.

关 键 词：铅 TCR VΒ基因 基因扫描 T细胞克隆性 

分 类 号：R135.12[医药卫生—劳动卫生]