Mild moxibustion at Tianshu (ST 25) decreases expression of prokineticin-1 and prokineticin receptor-1 in colon tissue of rats with chronic visceral hyperalgesia  

作　　者：Luyi Wu Chunhui Bao Linbao Ge Cili Zhou Huirong Liu Li Qi Tao Yi Huangan Wu Xiaomei Wang 

机构地区：[1]Qigong Institute, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China [2]Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

出　　处：《Neural Regeneration Research》2011年第33期2600-2604,共5页中国神经再生研究（英文版）

基　　金：the National Basic Research Program of China (973 program), No.2009CB522900;Shanghai Leading Academic Discipline Project, No. S30304; National Natural Science Foundation of China, No.81001549; Specialized Research Fund for the Doctoral Program of Higher Education, No.200802680001

摘　　要：Prokineticin-1 and prokineticin receptor-1 play important roles in visceral hypersensitivity and in-flammatory pain. Visceral hypersensitivity is closely associated with irritable bowel syndrome. Mild moxibustion can relieve chronic visceral hyperalgesia in rats with irritable bowel syndrome. We hypothesized that prokineticin-1 and prokineticin receptor-1 is the key target in the mechanism. This study established chronic visceral hyperalgesia rat models by colorectal distention. Protein and mRNA expression of prokineticin-1 and prokineticin receptor-1 were determined by immunohisto-chemical method and fluorescence quantitative-PCR, respectively, and were found to be signifi-cantly increased in visceral hyperalgesic rats. Mild moxibustion at Tianshu （ST 25） decreased prokineticin-1 and prokineticin receptor-1 expression in chronic visceral hyperalgesia rats and lessen the chronic visceral hyperalgesia in rats with irritable bowel syndrome at different levels of colorectal distention pressure.Prokineticin-1 and prokineticin receptor-1 play important roles in visceral hypersensitivity and in-flammatory pain. Visceral hypersensitivity is closely associated with irritable bowel syndrome. Mild moxibustion can relieve chronic visceral hyperalgesia in rats with irritable bowel syndrome. We hypothesized that prokineticin-1 and prokineticin receptor-1 is the key target in the mechanism. This study established chronic visceral hyperalgesia rat models by colorectal distention. Protein and mRNA expression of prokineticin-1 and prokineticin receptor-1 were determined by immunohisto-chemical method and fluorescence quantitative-PCR, respectively, and were found to be signifi-cantly increased in visceral hyperalgesic rats. Mild moxibustion at Tianshu （ST 25） decreased prokineticin-1 and prokineticin receptor-1 expression in chronic visceral hyperalgesia rats and lessen the chronic visceral hyperalgesia in rats with irritable bowel syndrome at different levels of colorectal distention pressure.

关 键 词：mild moxibustion irritable bowel syndrome chronic visceral hyperalgesia prokineticins neural regeneration 

分 类 号：S858.966[农业科学—临床兽医学] Q426[农业科学—兽医学]