传染性单核细胞增多症患儿外周血EBV-DNA载量与T细胞亚群的相关性分析  

Correlated Analysis of Epstein-Barr Virus Load and Changes of T Cell Subsets in Children with Infectious Mononucleosis

作　　者：郭娟娟[1] 常虹[2] 郝国平[2] 王晓欢[2] 朱镭[3] 

机构地区：[1]山西医科大学,山西太原030001 [2]山西省儿童医院血液科,山西太原030000 [3]山西省儿童医院检验科,山西太原030000

出　　处：《中国现代医生》2011年第34期1-2,5,共3页China Modern Doctor

摘　　要：目的应用流式细胞术检测传染性单核细胞增多症(IM)患者与正常儿童T细胞亚群、Treg细胞的百分比;分析IM患者急性期外周血EB病毒DNA浓度与它们的相关性。方法选取确诊为儿童传单且外周血EBV-DNA阳性的急性期患者47例,同期正常儿童32例作为对照,比较两组患者外周血T淋巴细胞亚群CD3+、CD4+、CD8+、CD4+/CD8+及CD4+CD25+、CD4+CD25+CD127-的变化,分析它们与EBV浓度的相关性。结果 IM组患者与对照组相比急性期CD3+、CD8+T细胞百分比明显上升,CD4+、CD4+/CD8+比值、CD4+CD25+、CD4+CD25+CD127-下降。在急性期IM患者组病毒载量与CD3+、CD8+T细胞均呈正相关;与CD4+、CD4+/CD8+、CD4+CD25+、CD4+CD25+CD127-细胞呈负相关。结论传染性单核细胞增多症患者急性期存在细胞免疫及免疫调节功能紊乱,其免疫功能紊乱的程度与EB病毒感染量有关,为临床IM患儿的治疗提供了理论依据。调节性T细胞在IM患儿急性期减少,可能在IM的发病机制中起了重要的作用。Objective To measure the expression level of T lymphocyte subsets in children with infectious mononucleosis（IM） during acute phase;To study the correlation of Epstein-Barr Virus load and changes of T cell subsets in children with IM. Methods T lymphocyte subsets and regulation T cells in peripheral blood were measured by flow cytometry in 47 cases with acute infectious mononucleosis. The measured results were compared with that of 32 normal children. Results Compared with the following results in controls, CD3＋,CD3＋CD8＋ were increased and CD3＋CD4＋,CD4＋/CD8＋ ratio,CD4＋CD25＋,CD4＋CD25＋CD127- were reduced in the IM patients at the acute stage. There was a positive correlation between EBV loaf and CD3＋,CD8＋ during acute phase. There was a negative correlation between EBV load and CD4＋ and CD4＋/CDS＋ratio,regulation T cells during acute phase. Conclusion There lies the immune dysfunctions in the children with acute infectious mononucleosis. The degree of immune dysfunction is relevant to EBV-DNA copy number. Meanwhile,regulation T cells of peripheral blood in the IM patients was diminished,they possible play an important role in the pathogenesis of IM.

关 键 词：传染性单核细胞增多症 T淋巴细胞亚群 流式细胞仪 EB病毒DNA CD4+CD25+CD127- 

分 类 号：R725.1[医药卫生—儿科]