δ阿片受体激动剂DADLE对脓毒症大鼠肺组织高迁移率族蛋白1水平的影响  

Effect of Delta-opioid Receptor Agonist on Lung Tissue HMGB1 Level in A Rat Model with Sepsis

作　　者：陶玉龙[1] 唐成武[1] 冯文明[1] 鲍鹰[1] 朱鸣[1] 

机构地区：[1]湖州师范学院附属第一医院肝胆外科分子外科研究所,浙江湖州313000

出　　处：《中国现代医生》2011年第34期8-9,共2页China Modern Doctor

基　　金：浙江省湖州市自然科学基金资助项目(编号:2009YZ12)

摘　　要：目的研究δ阿片受体激动剂DADLE(D-Ala2-D-Leu5-enkephalin)对脓毒症大鼠肺组织高迁移率族蛋白1(HMGB1)的水平的影响。方法将96只SD大鼠分为假手术组(SO)、脓毒症组(SEP)、DADLE组[制模后立即给药DADLE(5mg/kg,腹腔内注)],每组32只。采用改良盲肠结扎穿孔方法(CLP)建立大鼠脓毒症模型,于制模后6h、12h、18h和24h每组各取8只处死取材,Western Blot法检测肺组织HMGB1水平。结果 12h时SEP组和DADLE组HMGB1水平与SO组相比均明显升高(P<0.05),且DADLE组明显低于SEP组(P<0.05);18h时,SEP组和DADLE组HMGB1水平达到最高,同样,DADLE组明显低于SEP组(P<0.05);24h时SEP组和DADLE组HMGB1水平较18h有所降低,但DADLE组仍明显低于SEP组(P<0.05)。结论 DADLE能明显降低脓毒症大鼠肺组织HMGB1水平。Objective To study the effect of deha-opioid receptor agonist （DADLE）on lung tissue HMGB1 level in rat with sepsis. Methods Ninty-six SD rats were randomly divided into sham operated group （SO）,septic group （SEP）and DADLE group（DADLE）. Septic model was produced by cecal ligation and puncture （CLP）. In SO group,the abdomen was opened without any other treatment. In DADLE group,DADLE was administrated at a dose of 5mL/kg by intra-peritoneal injection after CLP. Rats were sacrificed at 6h,12h, 18h and 24h after CLP. Lung tissue HMGB1 level was determined by Western Blot. Results Lung tissue HMGB1 levels of SEP and DADLE group began to increase at 12h and reached the peak around 18h after CLP. Notably,lung tissue HMGB1 levels of DADLE group at 12h,18h and 24h were significantly lower than those of SEP group （P〈0.05）. Conclusion DADLE could significantly decrease lung tissue HMGB1 level in a rat model of sepsis.

关 键 词：DADLE 脓毒症 高迁移率族蛋白1 

分 类 号：R631[医药卫生—外科学]