氟比洛芬酯对大鼠脑缺血再灌注损伤后血浆血栓素A_2和前列环素I_2水平的影响  

Effect of flurbiprofen axetil on plasma thromboxane A_2 and prostacycline I_2 after cerebral ischemia-reperfusion injury in rats

作　　者：汤汝 浦少锋[2] 郭婷 

机构地区：[1]浙江桐庐县第一人民医院麻醉科,桐庐311500 [2]上海交通大学附属第六人民医院麻醉科,上海200233

出　　处：《中国临床药学杂志》2011年第6期344-346,共3页Chinese Journal of Clinical Pharmacy

摘　　要：目的探讨非选择性环氧化酶抑制剂氟比洛芬酯对大鼠局灶性脑缺血再灌注损伤后血浆血栓素A_2(TXA_2)和前列环素I_2(PGI_2)水平的影响。方法采用线栓法建立大鼠大脑中动脉闭塞2 h/再灌注24 h动物模型。24只♂SD大鼠随机分成4组,每组6只:假手术组、生理盐水对照组、氟比洛芬酯6和12 mg·kg^(-1)组。于插入线栓前10 min分别给予2mL生理盐水、氟比洛芬酯6和12 mg·kg^(-1)。大脑中动脉闭塞2h/再灌注24 h后采集大鼠血浆标本,放射免疫法检测血浆TXA_2的代谢产物血栓素B_2(TXB_2)和PGI_2的代谢产物6-酮-前列腺素_(1α)(6-keto-PGF_(1α))浓度。结果大脑中动脉闭塞2h/再灌注24 h后对照组与假手术组相比,TXB_2浓度显著增加[(1 038.12±110.02)ng·L^(-1) vs(341.29±47.98)ng·L^(-1),P<0.01],6-keto-PGF_(1α)显著减少[(539.79±45.07)ng·L^(-1) vs(1 268.24±131.01)ng·L^(-1),P<0.01]。氟比洛芬酯6及12 mg·kg^(-1)组与对照组相比能显著降低血浆TXB_2浓度[(605.53±45.27)、(495.46±66.03)ng·L^(-1),P<0.01],增加血浆6-keto-PGF_(1α)浓度[(839.34±60.22)、(963.08±82.86)ng·L^(-1),P<0.01]。氟比洛芬酯12 mg·kg^(-1)比6 mg·kg^(-1)效果更为显著。结论氟比洛芬酯能减轻大鼠脑缺血再灌注损伤后血浆TXA_2和PGI_2的变化。AIM To investigate the effect of nonselective cyclooxygenase inhibitor flurbiprofen axetil （FPA） on the plasma thmmboxane A2 （TXA2） and pmstacycline I2 （PGI2） in rats after fecal cerebral ischemia repeffusion injury （CIRI）. METHODS h model of occlusion of the middle cerebral artery （MCAO） 2h/reperfusion 24 h was used. Twenty-four SD male rots were randomly divided into 4 groups： sham, control, FPA 6 mg＇kg-1 and FPA 12 mg＇kg-1 groups. Each group had 6 rats and they were treated with 2 mL normal saline,6 mg＇kg-1FPA and 12 mg＇kg-1FPA re- spectively 10 min before the suture-embolus insertion. After 2 h of occlusion and 24 h of reperfusion, the plasma TXB2（a metabolite of TXA2） and 6-keto-PGFl,（a metabolite of PGI2）were measured by radioimmunoassay. RESULTS After 2 h of MCAO/24 h of repeffusion, control group had a higher plasma TXB2 level[ （1 038.12 ~ 110.02） vs （341.29 ＋ 47.98） ng＇L-1, P 〈 0.01] and a lower level of 6-keto-PGFl~[ （539.79 ~ 45.07） vs （1 268.24 ＋ 131.01）ng＂ L-1, P 〈0.01 ] as compared with Sham group. FPA 6 mg＇kg-1 and 12 mg＇kg-1 could significantly decrease the serum TXB2level [（605.53~45.27） and （495.46~66.03） vs （1 038.12＋ 110.02） ng-L-1,P 〈0.01] and increase the serum 6-keto-PGF1, level[ （839.34 ~ 60.22） and （963.08 ~ 82.86） vs （539.79 ~ 45.07） ng＂ L- l, p 〈 0.01 ] as compared with normal saline, q＇ne FPA dosage of 12 mg＂ kg-1 was more potent than that of 6 rag＂ kg-1. CONCLUSION FPA can attenuate the changes of TXA2 and PGI2 in rat plasma after focal CIRI.

关 键 词：氟比洛芬酯 血栓素A2 前列环素I2 脑缺血再灌注损伤 

分 类 号：R965[医药卫生—药理学]