Lithium chloride ameliorates learning and memory ability and inhibits glycogen synthase kinase-3 beta activity in a mouse model of fragile X syndrome  

作　　者：Shengqiang Chen Xuegang Luo Quan Yang Weiwen Sun Kaiyi Cao Xi Chen Yueling Huang Lijun Dai Yonghong Yi 

机构地区：[1]Department of Human Anatomy and Neurobiology, College of Basic Medicine, Central South University, Changsha 410013, Hunan Province, China [2]Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province, the Ministry of Education, Institute of Neuroscience, Second Affiliated Hospital of Guangzhou Medical College, Guangzhou 510260, Guangdong Province, China [3]Laboratory Animal Research Center of Guangzhou Medical College, Guangzhou 510182, Guangdong Province, China

出　　处：《Neural Regeneration Research》2011年第31期2452-2459,共8页中国神经再生研究（英文版）

基　　金：the National Natural Science Foundation of China,No.30870876;the Natural Science Foundation of Guangdong Province,No.815101700100005;the Science and Technology Program of Guangdong Province,No.2005B60302004,2008B030301371,2009B030801368;the Traditional Chinese Medicineand Combination of Traditional Chinese and Western Medicine Program of Guangzhou,No.2008A52;the Medical and Health Scientific Research Program of Guangzhou,No.2009-YB-167

摘　　要：In the present study,Fmr1 knockout mice （KO mice） were used as the model for fragile X syndrome.The results of step-through and step-down tests demonstrated that Fmr1 KO mice had shorter latencies and more error counts,indicating a learning and memory disorder.After treatment with 30,60,90,120,or 200 mg/kg lithium chloride,the learning and memory abilities of the Fmr1 KO mice were significantly ameliorated,in particular,the 200 mg/kg lithium chloride treatment had the most significant effect.Western blot analysis showed that lithium chloride significantly enhanced the expression of phosphorylated glycogen synthase kinase 3 beta,an inactive form of glycogen synthase kinase 3 beta,in the cerebral cortex and hippocampus of the Fmr1 KO mice.These results indicated that lithium chloride improved learning and memory in the Fmr1 KO mice,possibly by inhibiting glycogen synthase kinase 3 beta activity.In the present study,Fmr1 knockout mice （KO mice） were used as the model for fragile X syndrome.The results of step-through and step-down tests demonstrated that Fmr1 KO mice had shorter latencies and more error counts,indicating a learning and memory disorder.After treatment with 30,60,90,120,or 200 mg/kg lithium chloride,the learning and memory abilities of the Fmr1 KO mice were significantly ameliorated,in particular,the 200 mg/kg lithium chloride treatment had the most significant effect.Western blot analysis showed that lithium chloride significantly enhanced the expression of phosphorylated glycogen synthase kinase 3 beta,an inactive form of glycogen synthase kinase 3 beta,in the cerebral cortex and hippocampus of the Fmr1 KO mice.These results indicated that lithium chloride improved learning and memory in the Fmr1 KO mice,possibly by inhibiting glycogen synthase kinase 3 beta activity.

关 键 词：fragile X syndrome Fmr1 knockout mice step-down test step-through test learning and memory glycogen synthase kinase 3 beta lithium chloride 

分 类 号：Q754[生物学—分子生物学] TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]