益气养阴消癥通络中药对早期糖尿病大鼠肾脏的保护作用及其相关机制  

Protective effect on kidney and mechanism of Chinese medicinal with actions of replenishing qi,nourishing yin,removing accumulation and free collateral vessels in rats with early diabetes

作　　者：陈志强[1] 赵雯红[1] 张江华[1] 孙玉凤[1] 王月华[1] 

机构地区：[1]河北医科大学,河北050017

出　　处：《北京中医药大学学报》2011年第11期746-750,I0001,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：河北省自然科学基金资助项目(No.C2008001074);河北省卫生厅资助项目(No.08165);河北省科学研究与发展计划项目(No.09276101D-27)

摘　　要：目的探讨益气养阴消癥通络中药对早期糖尿病大鼠肾脏的保护作用及其可能的分子生物学作用机制。方法将SD大鼠随机分为6组:单纯肾切组,模型组,厄贝沙坦组,中药低、中、高剂量组,按各组相应药物剂量灌胃。于给药第6周末检测各组大鼠24 h尿蛋白(UPro)量及肾功能水平;光镜下观察各组大鼠肾脏的病理改变;Western blot方法检测各组大鼠肾皮质p38丝裂原活化蛋白酶(p38MAPK和p-p38MAPK)、信号转导子和转录激活子(STAT3和p-STAT3)的蛋白表达水平。结果厄贝沙坦和中药低、中剂量组与模型组比较,Upro量及p-p38MAPK、p-STAT3的蛋白表达水平均显著下降(P<0.05);各治疗组与模型组比较,肾功能明显改善(P<0.05),病理改变有所减轻。结论抑制p38MAPK/STAT3信号转导通路的活化可能是益气养阴消癥通络中药对早期糖尿病大鼠肾脏保护作用的机制之一。Objective To investigate the protective effect of Chinese medicinal with the actions of replenishing qi,nourishing yin,removing accumulation and free collateral vessels on kidney in rats with early diabetes,and discuss possible molecular biology mechanism.Methods SD rats were randomly divided into the nephrectomy group,model group,irbesartan group,high-dose,mid-dose and low dose Chinese medicinal groups（high-dose group,mid-dose group and low-dose group）.All groups were given relevant medicinal intragastrically.At the end of the 6th week the levels of 24-hour urinary protein（U Pro） and renal function were detected in rats of all groups.The pathological changes of kidney were observed with light microscope.The protein expressions of p38MAPK,p-p38MAPK,STAT3 and p-STAT3 in renal cortex were detected by using Western blotting method.Results The level of U Pro and protein expressions of p-p38MAPK and p-STAT3 decreased significantly in the irbesatan group,low-dose group and mid-dose group compared with the model group（P0.05）.The renal function was improved and the pathological changes were relieved in all treatment groups compared with the model group（P0.05）.Conclusion The inhibition of activation of p38MAPK/STAT3 signal transmission pathway may be one of kidney-protecting mechanisms of Chinese medicinal with the actions of replenishing qi,nourishing yin,removing accumulation and free collateral vessels in rats with early diabetes.

关 键 词：益气养阴消癥通络中药 糖尿病肾病 作用机制 大鼠 

分 类 号：R285.5[医药卫生—中药学]