西藏核桃优良单株的FISH-AFLP分析  

FISH-AFLP analysis of walnut advanced trees in Tibet,southwestern China

作　　者：马庆国[1] 王滑[1,2] 潘刚[3] 裴东[1] 

机构地区：[1]中国林业科学研究院林业研究所,林木遗传育种国家重点实验室,100091 [2]华中农业大学园艺林学学院 [3]西藏农牧学院西藏高原生态研究所

出　　处：《北京林业大学学报》2011年第6期86-89,共4页Journal of Beijing Forestry University

基　　金：林业公益性行业科研专项(201004048)

摘　　要：为了深入了解西藏核桃种质资源的遗传背景和遗传多样性,采用FISH-AFLP技术,筛选9对EcoRⅠ+3和MseⅠ+3引物组合,对我国西藏核桃主产区加查和郎县的11个核桃(JuglansregiaL.)优良单株进行基因组DNA水平检测。结果表明:在获得的1103条可统计条带中,1076条呈多态性,多态性达97.67%;9对引物所检测到的不同位点的有效等位基因数(Ne)范围为1.00～1.99,平均为1.48±0.04;基因多样度(H)为0.00～0.49,平均为0.29±0.02;Shannon信息指数(I)为0.00～0.69,平均为0.44±0.02。本研究分析了西藏核桃优良单株的遗传多样性和亲缘关系,建立了它们的指纹图谱。In order to deeply understand the genetic background and the genetic diversity of Tibet walnut germplasm resources,FISH-AFLP was conducted using 9 EcoRⅠ＋3/MseⅠ＋3 primer combinations to analyse 11 walnut（Juglans regia L.） advanced trees from Tibet at genomic DNA level.Totally 1 103 AFLP fragments were obtained,in which 1 076 were polymorphic and the average percentage of polymorphic bands（ppb） was 97.67%.The value of effective number of alleles（Ne） ranged from 1.00 to 1.99 with an average of 1.48±0.04;Nei＇s gene diversity（H） ranged from 0.00 to 0.49 with an average of 0.29±0.02 and Shannon＇s information index（I） ranged from 0.00 to 0.69 with an average of 0.44±0.02.The genetic diversity and sibship among walnut advanced trees in Tibet were investigated and their fingerprintings were established in the research.

关 键 词：核桃 遗传多样性 亲缘关系 AFLP 指纹图谱 

分 类 号：S718.46[农业科学—林学]