解除紫椴种子休眠技术的研究  

Seed treatments to overcome dormancy of Tilia amurensis

作　　者：杨立学[1] 王海南[2] 范晶[3] 那守海[2] 

机构地区：[1]东北林业大学林学院 [2]东北林业大学野生动物资源学院 [3]黑龙江大学农业资源与环境学院

出　　处：《北京林业大学学报》2011年第6期130-134,共5页Journal of Beijing Forestry University

基　　金：黑龙江省林业科技项目(GA09B202-06);东北林业大学青年拔尖人才支持计划(YTTP-1011-14)

摘　　要：研究了紫椴种子的休眠与萌发条件,主要对其解除休眠的方法进行了研究。试验设计包括3种处理:不同时间的浓H2SO4处理(10、30、60min),4种不同的层积方式(无处理,0～5℃、30d,15～20℃、30d,15～20℃、10d→0～5℃、20d),不同浓度的GA3处理(0、100、300mg/L)。每种处理设置4个重复。经上述处理的种子最终萌发率与平均萌发时间与对照相比显著提高;同时,15～20℃、10d→0～5℃、20d的变温层积比其他恒温层积更加有效;层积处理效果比浓H2SO4、GA3的处理效果更好。对紫椴种子处理的最佳组合是以浓H2SO4浸泡种子30min,然后施以100mg/L的GA3进行处理,再进行15～20℃、10d→0～5℃、20d的层积,发芽率高达90%。The present research was conducted to study the dormancy breaking treatments in Tilia amurensis seed.An experiment was conducted with four replications and three treatments,including three different concentrated sulfuric acid treatments（10,30 and 60 min）,varying concentrations of GA3（0,100 and 300 mg/L） and 4 levels of stratification（0;0-5 ℃,30 d;15-20 ℃,30 d and 15-20 ℃,10 d→0-5 ℃,20 d）.The germination percentage and mean germination time of treated seeds were significantly enhanced by mentioned treatments compared with control.It was concluded that altering temperature stratification was more effective on studied dormancy breaking traits than others.Moreover,cold stratification was a better treatment on breaking seed dormancy of T.amurensis seeds than concentrated H2SO4and GA3 treatments.Applying concentrated sulfuric acid for 30 min and 100 mg/L concentration of GA3 and stratification（15-20 ℃,10 d→0-5 ℃,20 d） results in higher germination in T.amurensis dormant seeds,and germination percentage reaches 90%.

关 键 词：紫椴 种子 H2SO4 GA3 层积催芽 解除休眠 

分 类 号：S723.1[农业科学—林木遗传育种]