多胺含量及精胺和亚精胺对盐胁迫下平邑甜茶膜脂过氧化的影响  

Polyamine content and effects of spermine and spermidine on lipid peroxidation induced by salt stress in Malus hupehensis Rehd

作　　者：李强[1] 杨洪强[1] 沈伟[1] 

机构地区：[1]山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,国家苹果工程技术中心,271018

出　　处：《北京林业大学学报》2011年第6期173-176,共4页Journal of Beijing Forestry University

基　　金：国家自然科学基金项目(30571285、30671452);山东省中青年科学家奖励基金项目(2008BS07006)

摘　　要：以一年生平邑甜茶幼苗为材料,在水培条件下研究了盐胁迫下平邑甜茶叶片内源游离态多胺、丙二醛(MDA)含量的变化以及外源多胺和多胺合成抑制剂对它们的影响。结果表明:盐胁迫下平邑甜茶叶片中MDA含量显著增加,精胺(Spm)和亚精胺(Spd)含量明显降低,腐胺(Put)含量显著增加,(Spd+Spm)/Put显著降低,多胺总量降低;外施精胺和亚精胺明显降低了盐胁迫下叶片MDA的含量,而外施多胺合成抑制剂明显提高了盐胁迫下叶片MDA的含量,加重了盐胁迫下平邑甜茶的膜脂过氧化。精胺和亚精胺能够减轻盐胁迫所导致的平邑甜茶叶片膜脂氧化损伤。Changes in the content of endogenous polyamines and malondialdehyde（MDA） responding to salinity stress and effects of exogenous polyamines and the polyamine biosynthesis inhibitor on polyamines and malondialdehyde（MDA） content were investigated in leaves of 1-year-old Malus hupehensis Rehd.seedlings grown in hydroponic systems.Results showed that the content of free putrescine（Put） was increased,and the content of free Spd and Spm and（Spd＋Spm）/Put ratio in leaves were significantly decreased under salt treatment.Accumulation of MDA was significantly decreased treated with exogenous Spd or Spm under salt stress.Moreover,the polyamine biosynthesis inhibitor aggravated the lipid peroxidation caused by salt stress in M.hupehensis,and the content of MDA was increased markedly.It suggests that spermidine and spermine could alleviate the damages on lipid peroxidation induced by salt stress in M.hupehensis.

关 键 词：多胺 盐胁迫 丙二醛 

分 类 号：S718.43[农业科学—林学]