切口痛-瑞芬太尼痛觉过敏大鼠脊髓总蛋白及膜蛋白NMDA受体NRl、NR2A及NR2B亚基表达的变化  

Changes in total and surface N-methyl-D-aspartate receptor NR1, NR2A and NR2B subunits in spinal cord in a rat model of incisional pain-remifentanil.induced hyperalgesia

作　　者：元元[1] 王晶瑶[1] 苑方[1] 王海云[1] 于泳浩[1] 王国林[1] 

机构地区：[1]天津医科大学总医院麻醉科,300052

出　　处：《中华麻醉学杂志》2011年第9期1045-1048,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30972847）;天津市应用基础及前沿计划研究项目（08JCYBJCOSOOO）

摘　　要：目的评价切口痛．瑞芬太尼痛觉过敏大鼠脊髓总蛋白及膜蛋白NMDA受体NRl、NR2A及NR2B亚基表达的变化。方法尾静脉置管成功的雄性sD大鼠32只，体重240—260g，月龄2～3月，采用随机数字表法，将大鼠随机分为4组（n=8）：对照组（C组）静脉输注等容量生理盐水60min，瑞芬太尼组（R组）静脉输注瑞芬太尼1．2μg·kg-1·min-160min；切口痛组（I组）建立切I：1痛模型，同时静脉输注等容量生理盐水60min；瑞芬太尼＋切口痛组（R＋I组）建立切口痛模型，同时静脉输注瑞芬太尼1．2μg·kg-1·min-160min。于生理盐水或瑞芬太尼给药前24h、给药后2,6、24和48h时测定机械刺激缩足阈值（PWT）和热刺激缩足潜伏期（PWL），最后一次测定痛阈后处死取脊髓L4-6节段，采用Western blot法测定脊髓总蛋白及膜蛋白NMDA受体NRl、NR2A及NR2B亚基的表达，并计算膜蛋白中NP,2B／NR2A比值。结果与C组比较，I组、R组和R＋I组PWT降低，PWL缩短，总蛋白及膜蛋白NMDA受体NRl和NR2B亚基表达上调，膜蛋白中NR2B／NR2A比值增加（P〈0．05）。与R组和I组比较，R＋I组PWT降低，PWL缩短，总蛋白及膜蛋白NMDA受体NRl和NR2B亚基表达上调，膜蛋白中NR2B／NR2A比值增加（P〈0．05）。各组总蛋白及膜蛋白NMDA受体NR2A亚基表达差异无统计学意义（P〉0．05）。结论大鼠切口痛．瑞芬太尼痛觉过敏的形成可能与脊髓总蛋白及膜蛋白NMDA受体NRl和NR2B亚基表达上调和膜蛋白中NMDA受体NR2B亚基组成比例的增加有关。Objective To investigate the changes in total and surface N-methyl-D-aspartate receptor （NMDAR） NR1, NR2A and NR2B subunits in spinal cord in a rat model of incisional pain（IP）-remifentanil-induced hyperalgesia. Methods Thirty-two male SD rats in which caudal vein catheter were successfully placed were randomly divided into 4 groups （n = 8 each） ： control group（group C）, remifentanil group（group R）, IP group （group I） and IP＋ remifentanil group （group R ＋ I）. In groups R and C remifentanil 1.2 μg·kg-1·min-1 or the same volume of normal saline was infused intravenously for 60 min. In groups R ＋ I and I, a 1 cm longitudinal inci- sion was made in the plantar surface of left hindpaw and remifentanil μg·kg-1·min-1 or the same volume of normal saline was infused intravenously for 60 min. Paw withdrawal threshold （PWT） and paw withdrawal latency （PWL） were measured at 24 h before and 2, 6, 24, 48 h after remifentanil or normal saline administration. The animals were sacrificed after last pain threshold measurement. The L4-6 segment of the spinal cord was isolated for determination of the expression of the total and surface NMDAR NR1, NR2A and NR2B subunits in spinal cord by Western blot. The ratio of surface NR2B/NR2A was calculated. Results Compared with group C, PWT was sig- nificantly decreased, PWL shortened, the expression of the total and surface NMDAR NR1, NR2A and NR2B sub- units up-regulated and the ratio of surface NR2B/NR2A increased in groups I, R and R ＋ I（ P 〈 0.05）. Compared with groups R and I, PWT was significantly decreased, PWL shortened, the expression of the total and surface NMDAR NR1, NR2A and NR2B subunits up-regulated and the ratio of surface NR2B/NR2A increased in group R ＋ I（ P 〈 0.05） .There was no significant difference in the total and surface NMDAR NR2A subunits among the four groups（ P 〉 0.05）. Conclusion The up-regulation of total and surface NMDAR NR1 and NR2B subunits and the increased percentage of surface NR2B s

关 键 词：哌啶类 痛觉过敏 受体 N-甲基-D 天冬氨酸 脊髓 

分 类 号：R614[医药卫生—麻醉学]